相关实验视频

Updated: Jun 7, 2025

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
09:27

Functional Complementation Analysis FCA: A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways

Published on: June 24, 2016

17.6K

自然产品生物合成中的假定终端化域的结构

Michael R Rankin, Dheeraj Khare, Lena Gerwick

    bioRxiv : the preprint server for biology
    |November 18, 2024
    PubMed
    概括

    研究人员在细菌中特征了一种新的终端化域 (TAD). 这个域结合NAD,在产生化天然产品方面起着催化作用,扩大了已知的多基基合成酶和非核糖体合成酶的化学结构.

    更多相关视频

    A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products07:59

    A Customizable Approach for the Enzymatic Production and Purification of Diterpenoid Natural Products

    Published on: October 4, 2019

    9.7K
    From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü602809:08

    From a Natural Product to Its Biosynthetic Gene Cluster: A Demonstration Using Polyketomycin from Streptomyces diastatochromogenes Tü6028

    Published on: January 13, 2017

    17.1K

    相关概念视频

    Amino Acid Biosynthetic Pathways01:29

    Amino Acid Biosynthetic Pathways

    Amino acid biosynthesis is essential for cell growth, protein synthesis, and metabolic regulation. Cells generate essential and non-essential amino acids from metabolic intermediates to sustain vital biological functions. These intermediates originate from key metabolic pathways: glycolysis, the tricarboxylic acid (TCA) cycle, and the pentose phosphate pathway. Important precursors include α-ketoglutarate, pyruvate, oxaloacetate, phosphoenolpyruvate, and erythrose-4-phosphate, which...
    3
    Amines to Amides: Acylation of Amines01:19

    Amines to Amides: Acylation of Amines

    Various carboxylic acid derivatives (such as acid chlorides, esters, and anhydrides) can be used for the acylation of amines to yield amides. The reaction requires two equivalents of amines. The first amine molecule functions as a nucleophile and attacks the carbonyl carbon to produce a tetrahedral intermediate. This is followed by the loss of the leaving group and restoration of the C=O bond.
    Next, the second equivalent of amine serves as a Brønsted base and deprotonates the quaternary...
    2.4K
    Aldehydes and Ketones with Amines: Imine Formation Mechanism01:23

    Aldehydes and Ketones with Amines: Imine Formation Mechanism

    Imine formation involves the addition of carbonyl compounds to a primary amine. It begins with the generation of carbinolamine through a series of steps involving an initial nucleophilic attack and then several proton transfer reactions. The second part includes the elimination of water, as a leaving group, to give the imine.
    Imines are formed under mildly acidic conditions. A pH of 4.5 is ideal for the reaction.
    If the pH is low or the solution is too acidic, the reaction slows down in the...
    5.3K
    Peptidoglycan Synthesis01:28

    Peptidoglycan Synthesis

    Structure of PeptidoglycanPeptidoglycan is a vital structural component of the bacterial cell wall, providing mechanical strength and shape to the cell. It consists of repeating units of two sugars—N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM)—linked by β-1,4 glycosidic bonds. These sugar chains are cross-linked by short peptide chains, forming a mesh-like polymer that surrounds the bacterial plasma membrane.Cytoplasmic Phase – Precursor SynthesisPeptidoglycan...
    2
    Preparation of Amides01:29

    Preparation of Amides

    Amides are synthesized by treating carboxylic acids with amines in the presence of dehydrating agents like dicyclohexylcarbodiimide (DCC).
    The DCC-promoted synthesis of amides begins with the protonation of DCC by carboxylic acid. The protonation makes it a better acceptor. Next, the addition of carboxylate to the protonated carbodiimide gives a reactive acylating agent.
    Subsequently, the amine acts as a nucleophile that attacks the acylating agent to form a tetrahedral intermediate. In the...
    3.0K
    Acid Halides to Amides: Aminolysis01:07

    Acid Halides to Amides: Aminolysis

    Aminolysis is a nucleophilic acyl substitution reaction, where ammonia or amines act as nucleophiles to give the substitution product. Acid halides react with ammonia, primary amines, and secondary amines to yield primary, secondary, and tertiary amides, respectively.
    In the first step of the aminolysis mechanism, the amine attacks the carbonyl carbon of the acyl chloride to form a tetrahedral intermediate. In the second step, the carbonyl group is re-formed with the elimination of a chloride...
    2.6K