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相关概念视频

Immunodeficiency Diseases01:25

Immunodeficiency Diseases

909
Immunodeficiency disorders are conditions in which the immune system's ability to fight infectious disease and cancer is compromised or entirely absent. The immune system comprises a complex network of cells, tissues, and organs that work together to protect the body from potentially harmful invaders. When this system is deficient or not functioning properly, it leaves the body susceptible to infections, diseases, or other complications.
There are three main causes of immunodeficiency...
909
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

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Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
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Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

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All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
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相关实验视频

Updated: Jun 7, 2025

Isolation and Primary Culture of Mouse Aortic Endothelial Cells
08:20

Isolation and Primary Culture of Mouse Aortic Endothelial Cells

Published on: December 19, 2016

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免疫抑制药物对内皮细胞功能表现出不同的影响.

Aly Elezaby, Ryan Dexheimer, David Wu

    bioRxiv : the preprint server for biology
    |November 18, 2024
    PubMed
    概括

    常见的免疫抑制药物sirolimus和tacrolimus对内皮细胞功能的影响不同. 西洛利斯会影响细胞迁移,增殖和血管生成,而塔克罗利斯会影响氧化的释放,为药物诱导的血管问题提供了洞察力.

    科学领域:

    • 血管生物学 血管生物学
    • 免疫药理学 免疫药理学
    • 干细胞生物学 干细胞生物学

    背景情况:

    • 免疫抑制药物对于管理瘤,自身免疫性疾病和器官移植至关重要.
    • 这些药物与不良的血管改造有关,但细胞特异性影响尚不清楚.
    • 了解药物诱导的内皮功能障碍对于患者的治疗结果至关重要.

    研究的目的:

    • 研究免疫抑制药物对内皮细胞功能的差异性影响.
    • 为了利用诱导多能干细胞衍生内皮细胞 (iPSC-ECs) 作为模型系统.
    • 阐明药物相关血管并发症背后的机制.

    主要方法:

    • 培养iPSC-ECs以建模内皮细胞.
    • 使用常见的免疫抑制药物,包括西罗和塔克罗.
    • 评估内皮细胞功能:迁移,增殖,乙化LDL吸收,血管生成和氧化释放.

    主要成果:

    • 赛洛斯显著降低了内皮细胞迁移,增殖,乙化LDL吸收和血管生成.
    • 塔克罗利斯主要抑制了内皮细胞释放的氧化.
    • iPSC-ECs对各种免疫抑制剂表现出不同的敏感性.

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    Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model
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    Author Spotlight: Magnetic Bead-Based Isolation of Murine Dermal Lymphatic Endothelial Cells
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    Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model
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    Generation of an Immortalized Murine Brain Microvascular Endothelial Cell Line as an In Vitro Blood Brain Barrier Model

    Published on: August 29, 2012

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    结论:

    • 免疫抑制药物对内皮细胞功能表现出明显的影响.
    • 与塔克罗利斯相比,西罗利斯对基本内皮细胞功能构成更大的风险.
    • 这种iPSC-EC模型有助于研究药物特异性血管毒性.