在施万细胞中,STRIPAK复合体是辐射分类和胺受体表达所必需的
bioRxiv : the preprint server for biology
|November 18, 2024
概括
类蛋白对外周神经系统发育至关重要,作为新的Rac1相互作用体. 这项研究揭示了它们在施万细胞细胞骨组织和Hippo通路调节中的作用.
科学领域:
- 神经科学是一个神经科学.
- 细胞生物学 细胞生物学
- 发展生物学 发展生物学
背景情况:
- 施万细胞对于外周神经系统的发育至关重要,需要Rac1依赖的细胞骨变化来进行轴突结.
- 在这个过程中,Rac1的直接下游影响者仍然不完全理解.
研究的目的:
- 确定参与施万细胞功能中的新型Rac1相互作用体.
- 阐明状蛋白和Rac1在施万细胞发育和希波细胞通路调节中的作用.
主要方法:
- 酵母双混合选以识别Rac1相互作用体.
- 在小鼠中,施瓦恩细胞特异性基因切除 (Rac1,条蛋白-3,条蛋白-1/3).
- 对细胞骨组织的分析,拉梅利波迪亚的形成和辐射分类.
- 对于Hippo通路组分酸化 (YAP/TAZ) 的西部涂抹.
主要成果:
- 确定了striatin-3和MOB4作为新的Rac1相互作用体.
- 在 Schwann 细胞中消除条性素-3 损害了类似于 Rac1-null 细胞的 lamellipodia 形成.
- 多个条纹蛋白质的有条件淘汰严重延迟了辐射分类.
- 删除Rac1或素-1/3破坏了Hippo通路调节,YAP/TAZ酸化和基因表达.
结论:
- 斯特里亚丁-3是一种新的Rac1相互作用因子,对于施万细胞状体的形成至关重要.
- 类蛋白对于适当的外周神经系统发育,包括辐射分类,是必需的.
- Rac1 调节了 Schwann 细胞中的 Hippo 途径,影响了 YAP/TAZ 活动和基因表达.
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