使用Elaeagnus angustifolia的L.面粉作为封装材料,通过乳液方法封装Lacticaseibacillus casei和Lactobacillus acidophilus
Büşra Karkar1, Saliha Şahin1, Lütfiye Yılmaz-Ersan2
1Faculty of Science and Arts, Department of Chemistry University of Bursa Uludağ Bursa Türkiye.
Food science & nutrition
|November 18, 2024
概括
油面粉成功封装了Lacticaseibacillus casei和Lactobacillus acidophilus的益生菌. 与自由细菌相比,封装细菌在体外消化和储存过程中显示出增强的生存能力.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 益生菌细菌如Lacticaseibacillus casei和Lactobacillus acidophilus提供健康益处,但对消化敏感.
- 油面粉富含化合物和益生菌,是潜在的天然封装材料.
- 开发有效的封装方法对于提高益生菌生存率和有效性至关重要.
研究的目的:
- 为了优化 Lacticaseibacillus casei 和 Lactobacillus acidophilus 的封装,使用油面粉.
- 评估封装益生菌的物理化学特性和结构完整性.
- 评估封装益生菌的储存稳定性和体外胃肠道存活率.
主要方法:
- 采用中央复合材料设计响应表面方法来优化封装条件.
- 扫描电子显微镜 (SEM) 用于确定囊大小.
- 福里埃变换红外光谱法 (FTIR) 分析了封装后油面粉的结构完整性.
- 进行了体外胃肠道消化和储存稳定性测试.
主要成果:
- 最佳封装产生了高效率:L. casei的93.66%,L. acidophilus的74.97%.
- 囊大小被确定为L. casei大约104.8微米,L. acidophilus大约95.7微米.
- 在封装后,FTIR分析证实油面粉没有发生结构变化.
- 与自由益生菌相比,封装益生菌在储存期间显著减少活力损失,在体外胃肠道消化后生存率显著提高 (39.59%的L. casei,L. acidophilus的36.28%) 无益生菌.
结论:
- 油面粉是一种有效且适合封装Lacticaseibacillus casei和Lactobacillus acidophilus的材料.
- 封装显著提高了这些益生菌细菌的稳定性和胃肠道存活率.
- 这项研究表明,通过自然封装技术,改善益生菌的疗效是一种有希望的方法.
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