通过下一代测序来改善性真菌感染的诊断:在手术ICU患者中进行的一项试点研究
Sara Posadas-Cantera1, Negin Mehrbarzin1, Simon Wetzel1,2
1Institute of Medical Microbiology and Hygiene, Faculty of Medicine, Medical Center - University of Freiburg, Freiburg, Germany.
Frontiers in cellular and infection microbiology
|November 18, 2024
概括
与传统方法相比,下一代测序 (NGS) 在性液中诊断真菌感染的灵敏度更高. 这种先进的技术有助于识别病原体,改善了重症监护机构患者的治疗结果.
科学领域:
- 医学微生物学 医学微生物学
- 基因组学就是基因组学.
- 关键护理医学 关键护理医学
背景情况:
- 炎是一种与肝硬化或肠道穿孔相关的严重疾病,易患真菌感染.
- 炎的真菌感染显著增加死亡率,特别是在重症监护室 (ICU).
- 目前的微生物诊断在性液体中的真菌病原体缺乏灵敏度,需要改进方法.
研究的目的:
- 评价下一代测序 (NGS) 的有效性,用于识别酸性液体中的真菌病原体.
- 将NGS的诊断性能与传统的基于培养的方法进行比较.
主要方法:
- 预期从怀疑感染的ICU患者中采集50个性流体样本.
- 标准微生物学测试 (培养) 和真菌DNA分离,然后进行NGS分析.
- 用于真菌识别的内部转录间隔器2 (ITS2) 区域的分析.
主要成果:
- 在50个样本中,NGS在19个样本中确定了真菌病原体,远远超过单独培养的样本.
- 检测到的培养物是Candida spp. 在只有五个样本中.
- 通过测序检测真菌DNA与较差的患者结果和特定的临床参数相关.
结论:
- 与标准培养相比,NGS在液中诊断真菌感染的灵敏度更高.
- 这种先进的诊断方法有望指导抗真菌疗法并改善重症监护患者的治疗结果.
- 标准化NGS工作流程和结果解释对于临床实施至关重要.
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