生物活性酸含有尿二甲基酸盐基复合材料的体内骨免疫反应:对聚甲基酸的潜在替代品) 骨水泥
Weerachai Singhatanadgit1, Piyarat Sungkhaphan2, Boonlom Thavornyutikarn2
1Faculty of Dentistry and Research Unit in Mineralized Tissue Reconstruction, Thammasat University (Rangsit Campus), Pathum-thani 12121, Thailand.
ACS materials Au
|November 18, 2024
概括
结合单酸单和氧阿帕的新复合骨凝土显示出对骨修复的前景. 这些材料支持骨细胞生长,同时抑制炎症和骨再吸收细胞.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 牙科材料 牙科材料
背景情况:
- 骨缺陷往往会在愈合过程中带来挑战,特别是当与炎症相关时.
- 目前的骨可能缺乏最佳骨质免疫调节所需的生物线索.
- 开发具有量身定制的生物特性的先进骨质水泥对于有效的骨再生至关重要.
研究的目的:
- 开发具有增强骨质免疫调节特性的新型复合骨.
- 研究将单酸单 (MCPM) 和酸 (HA) 结合在水泥性能上的影响.
- 为了在体外评估开发的水泥的生物相容性和细胞反应.
主要方法:
- 复合骨使用尿二甲酸盐 (UDMA),聚propylene glycol) 二甲酸盐 (PPGDMA) 和基乙烯二甲酸盐 (HEMA) 与酸玻璃填充剂进行合成.
- 加入MCPM和HA颗粒来提高生物性能.
- 通过使用启动剂 (过氧化 - BPO),激活剂 (N-tolyglycine glycidyl methacrylate - NTGGMA) 和抑制剂 (丁酸酸 - BHT) 来控制自由基聚合.
- 使用介质干细胞 (MSC),纤维细胞,RAW 246.7细胞和外周血液单核细胞 (PBMC) 来评估生物相容性.
主要成果:
- 添加BHT允许在25°C控制设置时间,在37°C减少延迟和增强转换.
- 复合水泥证明了与各种骨免疫细胞系的生物相容性.
- 含有MCPM和HA的水泥促进了MSC的生长和矿化,同时抑制了纤维细胞,骨质细胞原生体和PBMCs.
- 在实验室中观察到骨质母细胞分化,矿化,骨质母细胞发生和炎症反应的差异调节.
结论:
- 开发的复合骨表现出有希望的骨质免疫调节能力.
- MCPM和HA的组合有效地平衡骨形成并抑制炎症反应.
- 这些材料有可能用于骨修复应用,特别是用于管理与炎症相关的骨缺陷.
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