相关实验视频

Updated: Jun 7, 2025

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Identifying Mutations by High Resolution Melting in a TILLING Population of Rice

Published on: September 2, 2019

开发SNP基因类型测定试验,以确定大米中的标题日期

Noriyuki Kitazawa¹, Ayahiko Shomura¹, Tatsumi Mizubayashi¹

¹Institute of Crop Science, National Agriculture and Food Research Organization (NARO), 2-1-2 Kannondai, Tsukuba, Ibaraki 305-8518, Japan.

Breeding science

|November 18, 2024

概括

研究人员开发了41个米 (Oryza sativa L.) 中的标题日期 (HD) 位点的新型定型试验. 这些测试通过提供关键的HD基因型信息来改善作物,使得具有成本效益的标志物辅助育种成为可能.

科学领域:

农业学是一种农业学.
植物遗传学植物遗传学
分子育种是指分子育种.

背景情况:

标题日期 (HD) 是大米 (Oryza sativa L.) 的关键农学特征,影响地理和季节性适应.
了解HD基因型对于有效的标记器辅助育种计划至关重要.

研究的目的:

开发和验证在大米中的41个HD位点的高通量基因型测定试验.
评估这些HD位点的遗传多样性,跨越多样化的米品种.

主要方法:

利用Fluidigm 96-plex SNP基因造型平台,为41个HD位点 (29个基因,12个QTL) 创建测试.
基因定型377种大米品种,以确定等位基因和评估多样性.
开发了使用200种品种数据的HD预测模型,并对22种品种进行了验证.

主要成果:

成功开发的基因型鉴定试验区分了41个HD位置的144个等位基因.
观察到平均每位基因3.5个等位基因,在Hd1,Ghd7,PRR37和DTH8.8等关键位基因的多样性更高.
经过验证时,HD预测模型显示出良好的相关性 (r > 0.69,P < 0.001).

结论:

开发的基因型定型试验为HD提供了重要的基因型信息.
这些测定有助于提高米品种的具有成本效益和高效的标记器辅助育种.
这项研究增强了我们对大米中主要标题日期位置的遗传多样性的理解.

关键词:

日本的栽培品种.功能性核酸多态性多态性.基因型鉴定试验基因型鉴定试验标题日期标题日期标题日期米米饭米饭米饭米饭.单个核酸的多态性.

更多相关视频

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits

Published on: January 3, 2025

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes TILLING Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Published on: July 16, 2019

相关概念视频

Single Nucleotide Polymorphisms-SNPs

Single Nucleotide Polymorphisms-SNPs

A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...