Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.1K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.1K
Lineage Commitment01:21

Lineage Commitment

3.0K
Commitment is the  process whereby stem cells:
3.0K
Hematopoiesis01:21

Hematopoiesis

5.1K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
5.1K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.0K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.0K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

3.8K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
3.8K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Temperature-dependent functional inversion of Ca<sub>v</sub>3.2 channels during repetitive activity.

Molecular brain·2026
Same author

HSPCs and T<sub>reg</sub> cells cooperate to preserve extramedullary hematopoiesis under chronic inflammation.

Science advances·2026
Same author

Refractory infantile IPEX with Treg-restricted FOXP3null expression caused by a novel variant in <i>FOXP3</i>.

Journal of human immunity·2026
Same author

ACSL4-associated lipid metabolism is a distinct therapeutic vulnerability in KMT2A-rearranged acute myeloid leukemia.

Cell reports·2026
Same author

Condensin II mediates resistance to genotoxic stress and prevents mitotic defects in Arabidopsis.

Plant physiology·2026
Same author

Uncovering the glutamate carboxypeptidase II microenvironment using a multi-labeling proteomic approach.

Scientific reports·2025

相关实验视频

Updated: Jun 7, 2025

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.5K

WBP1L调节了造血干细胞功能和T细胞发育.

Imtissal Krayem1, Srdjan Grusanovic2, Iris Duric1,3

  • 1Laboratory of Leukocyte Signalling, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czechia.

Frontiers in immunology
|November 18, 2024
PubMed
概括

WW域结合蛋白1-like (WBP1L) 调节造血干细胞功能和白细胞的产生. 在小鼠中缺少它会导致失调的血液形成,扩大的甲状腺和改变的血细胞计数.

关键词:
在T细胞发育过程中,在WBP1L中使用.血液形成 血液形成造血干细胞和原始细胞移植造血干细胞 造血干细胞跨膜适配蛋白质的蛋白质适配器

更多相关视频

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

9.3K
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
12:03

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

18.6K

相关实验视频

Last Updated: Jun 7, 2025

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.5K
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

9.3K
Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
12:03

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors

Published on: July 8, 2012

18.6K

科学领域:

  • 血液学 血液学 血液学
  • 免疫学 免疫学 免疫学
  • 分子生物学分子生物学

背景情况:

  • WW域结合蛋白1-like (WBP1L),也被称为急性白血病1 (OPAL1) 的结果预测器,是一种跨膜适应蛋白.
  • WBP1L表达与ETV6-RUNX1转位和儿童白血病的良好预后相关.
  • 之前的研究发现WBP1L是CXCR4信号传递和血液形成的调节者.

研究的目的:

  • 研究WBP1L在调节血液形成和免疫细胞群中的作用.
  • 为了确定WBP1L缺乏对造血干细胞 (HSC) 和祖细胞的影响.
  • 分析WBP1L对胸膜发育和白细胞平衡的影响.

主要方法:

  • 在小鼠中删除了Wbp1l的生殖线.
  • 血液造血干细胞和原生细胞种群的分析.
  • 流细胞测量以评估胸细胞和外围血液细胞子集.
  • 造血干细胞和祖细胞移植实验.

主要成果:

  • Wbp1l生殖线删除导致HSC和早期祖先水平的失调血液形成.
  • 缺少WBP1L的小鼠表现出显著扩大的甲状腺,在所有亚群中增加了甲状腺细胞数量.
  • 在骨髓中增加了多能原始体4 (MPP4) 的生成,并观察到血液中白细胞数量增加.
  • WBP1L调节HSC功能和白细胞原生细胞的增殖,导致移植后增强WBP1L缺乏细胞的移植.

结论:

  • WBP1L是造血干细胞和原生细胞功能的关键调节者.
  • 在稳定状态条件下和骨髓移植后,WBP1L控制白细胞数量.
  • 调节不当的WBP1L会影响胸膜发育和周围血液细胞平衡.