两种新型乳球菌乳糖菌体vL_296和vL_20A的基因组表征
T A Chuksina1, A A Fatkulin1, N P Sorokina2
1Department of Biology and Biotechnology, HSE University, Moscow, 101000 Russian Federation.
Acta naturae
|November 18, 2024
概括
在奶酪乳清中发现了两种新的菌体物种vL_20A和vL_296. 这些菌体表现出独特的宿主范围,影响乳酸细菌对于发酵乳制品生产至关重要.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 基因组学就是基因组学.
背景情况:
- 发酵乳制品依赖于初始培养,主要是乳酸细菌.
- 这些细菌容易受到菌体感染,这可能会破坏生产.
- 菌体是感染细菌的病毒,在工业发酵中构成重大挑战.
研究的目的:
- 从奶酪乳清中分离和描述新型细菌菌物种.
- 确定新发现的菌体的物种,光谱和基因组特征.
- 研究这些菌体对乳制品生产中使用的初始培养物的潜在影响.
主要方法:
- 使用乳球球菌指标培养物分离细菌菌体.
- 对各种细菌宿主的菌体物种和光学光谱的确定.
- 双链DNA细菌菌体的基因组测序和分析.
- 与已知的菌体进行比较的基因组分析,包括*Lactococcus菌体bIL67*.
- 在初始培养基因组中分析菌素耐药性的CRISPR间隔器.
主要成果:
- 从奶酪乳清中分离出了两种新型细菌菌物种vL_20A和vL_296.
- 菌体vL_20A和vL_296具有不同的光学光谱,在21个测试中只共享4个共同宿主.
- vL_20A和vL_296的基因组分别为21,909和22,667个基对的线性双链DNA分子.
- *乳球菌bIL67*与新发现的菌具有很高的基因组相似性 (ANI 93.3-92.6).
- 在分析的初始培养物中没有发现针对vL_20A或vL_296的菌体特定CRISPR间隔器.
结论:
- 这项研究揭示了显著的生物多样性,以及乳制品环境中*Lactococcus lactis*菌体的广泛存在.
- 已识别的菌体,vL_20A和vL_296,表现出独特的宿主特异性和毒性.
- 细菌免疫系统,如CRISPR,有助于平衡对菌体毒性,影响起始培养的弹性.
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