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Updated: Jun 7, 2025

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
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A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

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开发基于 luciferase 的高度敏感的报告器,检测 ER 相关的蛋白质生物发生异常

Hiroshi Kadokura1,2,3, Nanshi Harada1, Satoshi Yamaki1

  • 1Institute of Multidisciplinary Research for Advanced Materials, Tohoku University, Sendai, Miyagi 980-8577, Japan.

iScience
|November 18, 2024
PubMed
概括

研究人员开发了一种敏感的火虫光酶 (FLuc) 记者,以检测细胞内膜网膜 (ER) 蛋白质折叠和局部化缺陷. 这个工具有助于理解分泌途径生物发生和ER氧化还原控制.

关键词:
生物工程是生物工程.细胞生物学 细胞生物学蛋白质结构方面 蛋白质结构方面

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相关概念视频

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Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
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