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Updated: Jun 7, 2025

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CIRCLE-Seq用于对非目标基因编辑进行审讯

Jeffrey Inen1, Chann Makara Han1, David M Farrel2

  • 1Department of Dermatology, University of Colorado School of Medicine, Anschutz Medical Campus; Gates Institute, University of Colorado School of Medicine, Anschutz Medical Campus.

Journal of visualized experiments : JoVE
|November 18, 2024
PubMed
概括

通过测序 (CIRCLE-seq) 进行切割效应体内报告的循环化识别了非故意的CRISPR-Cas9 DNA切割部位. 这种新的方法为基因组研究提供了敏感,高效和公正的非目标效应检测.

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