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相关概念视频

Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Operons02:09

Operons

Prokaryotes can control gene expression through operons—DNA sequences consisting of regulatory elements and clustered, functionally related protein-coding genes. Operons use a single promoter sequence to initiate transcription of a gene cluster (i.e., a group of structural genes) into a single mRNA molecule. The terminator sequence ends transcription. An operator sequence, located between the promoter and structural genes, prohibits the operon’s transcriptional activity if bound by a repressor...
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form dimers that...
Operon Model01:23

Operon Model

The operon model represents a fundamental mechanism of gene regulation in prokaryotes, enabling coordinated expression of genes involved in related metabolic or functional pathways. Operons consist of structural genes, a promoter, and an operator, with transcription regulated by repressors, activators, and small effector molecules.Structure and Function of OperonsAn operon is a cluster of structural genes transcribed together under the control of a single promoter. The promoter region...
Inducible Operons: lac Operon01:25

Inducible Operons: lac Operon

The lac operon in Escherichia coli is a model for understanding inducible gene regulation and metabolic flexibility. It integrates local control by lactose and global regulation through catabolite repression, enabling E. coli to preferentially metabolize glucose when available and switch to lactose utilization when glucose is scarce.Structure and Function of the lac OperonThe lac operon contains three structural genes: lacZ (β-galactosidase), lacY (lactose permease), and lacA (thiogalactoside...

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相关实验视频

Updated: May 8, 2026

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

Published on: August 21, 2016

兰巴抑制剂和兰巴Cro与兰巴操作员的同类相互作用.

A Hochschild, M Ptashne

    Cell
    |March 28, 1986
    PubMed
    概括
    此摘要是机器生成的。

    兰巴达抑制剂和Cro蛋白在菌体染色体上结合了相似的DNA位点. 这项研究表明,这两种蛋白质中的特定血清与相同的DNA位置接触,解释了它们独特的结合亲和力.

    更多相关视频

    CRISPR-Mediated Reorganization of Chromatin Loop Structure
    09:20

    CRISPR-Mediated Reorganization of Chromatin Loop Structure

    Published on: September 14, 2018

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
    08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    相关实验视频

    Last Updated: May 8, 2026

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System
    11:19

    Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

    Published on: August 21, 2016

    CRISPR-Mediated Reorganization of Chromatin Loop Structure
    09:20

    CRISPR-Mediated Reorganization of Chromatin Loop Structure

    Published on: September 14, 2018

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
    08:54

    In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

    Published on: March 29, 2019

    科学领域:

    • 分子生物学分子生物学
    • 遗传学 是一个遗传学.
    • 生物化学 生物化学

    背景情况:

    • 兰巴达抑制剂和Cro是蛋白质,它们与菌体染色体上的特定DNA序列结合.
    • 它们共有共同的结合点,但表现出不同的结合亲和力.
    • 在它们的识别螺旋中保存的氨基酸和在操作站点中的核酸表明了它们相互作用的结构基础.

    研究的目的:

    • 为了研究Lambda抑制器和Cro.中识别α螺旋体的2位保存胺的作用.
    • 为了确定这种血清残留物的特定DNA接触点.
    • 改进对压缩器和Cro如何识别具有不同亲和力的相似操作员站点的理解.

    主要方法:

    • 位点定向的突变发生改变了血清残留物.
    • 用DNA结合测试来测量结合亲和力.
    • 结构分析以确认蛋白质-DNA相互作用.

    主要成果:

    • 与之前的模型相反,在压缩器和Cro的识别螺旋的位置2的血清与运算器DNA的位置4的相同保存的核酸接触.
    • 这种互动解释了运营商网站的共享认可.
    • 其他相互作用的差异可能解释了它们明显的相对亲和关系.

    结论:

    • 保存的血清残留在兰巴抑制剂和Cro.的特定DNA识别中起着至关重要的作用.
    • 为这些蛋白质与相似的运算符序列的差异结合提出了一个简化的模型.
    • 这一发现增强了我们对蛋白质-DNA相互作用和序列识别机制的理解.