依赖的葡萄糖共同运输蛋白 (SGLTs) 不参与人类葡萄糖味道检测
R Kyle Palmer1, Anna B Nechiporenko1, Marc A Ilies2
1Opertech Bio, Inc., Philadelphia, Pennsylvania, United States of America.
PloS one
|November 18, 2024
概括
TAS1R2/R3受体,而不是像SGLT1这样的葡萄糖载体,在人类中介于甜味感知. 这项研究没有发现SGLT1替代途径在葡萄糖味道信号传递中的证据.
科学领域:
- * 神经科学是一门神经科学.
- * 感官生物学 * 感官生物学
- * 分子生物学 * 分子生物学
背景情况:
- *甜味感知主要归因于味觉受体细胞 (TRC) 中的TAS1R2/R3受体.
- *最近的研究表明,葡萄糖载体,特别是SGLT1,在可代谢糖的替代甜味途径中可能发挥作用.
- *研究这种替代途径对于理解甜味信号传递的完整机制至关重要.
研究的目的:
- * 调查-葡萄糖共运输体1 (SGLT1) 对人类葡萄糖味道信号的潜在贡献.
- * 确定SGLT1活性是否对于检测葡萄糖的甜味是必要的.
- * 为了澄清潜在的甜味感知的主要分子机制.
主要方法:
- *在中国仓鼠卵巢 (CHO) 细胞中表达的人类SGLT1 (hSGLT1),以测量葡萄糖介导的膜潜力变化.
- *使用葡萄糖进行度-反应分析,并测量了对SGLT抑制剂 (弗洛里津,米扎格利弗洛津) 和TAS1R2/R3对抗剂 (乳糖醇) 的反应.
- *与人类实验对象进行快速吞吐量味道歧视测定,以评估葡萄糖味道检测值和抑制剂的影响.
主要成果:
- * CHO细胞中的hSGLT1对葡萄糖有很高的亲和力 (EC50 = 452μM),被里津抑制 (IC50 = 3.5μM).
- *人类对葡萄糖的口味感知产生了较低的亲和度 (EC50 = 127-132 mM),表明转运体活动的机制不同.
- *TAS1R2/R3对手乳糖醇阻断了葡萄糖的味道,而SGLT1抑制剂里津和米扎格利弗洛津在相关度下没有影响葡萄糖的味道检测. 一些受试者可以检测到Phlorizin本身.
结论:
- *对TAS1R2/R3受体的激素激活足以解释人类的葡萄糖味觉.
- *没有证据支持SGLT1介导的替代信号通路有助于葡萄糖的味道.
- * 弗洛里津的味觉感知可能会混研究SGLTs在味觉中的作用.
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