使用协同作用的过氧化与分剂纳秒脉冲电场暴露的细菌的非活性化
Zachary Rosenzweig1, Jerrick Garcia1, Gary L Thompson2
1Department of Chemical Engineering, Rowan University, Glassboro, New Jersey, United States of America.
PloS one
|November 18, 2024
概括
将脉冲电场 (PEF) 与过氧化 (H2O2) 结合起来,显著增强了细菌的不活化. 这种非热性食品消毒方法实现了对大肠杆菌,L. innocua和S. enterica.的5分钟以上的减少.
科学领域:
- 食品科学与技术 食品科学与技术
- 微生物学 微生物学
- 非热处理的加工方法
背景情况:
- 脉冲电场 (PEF) 是一种用于食品消毒的非热技术.
- 将PEF与过氧化 (H2O2) 等化学剂相结合可能会提高效果.
研究的目的:
- 为了增强大肠杆菌,无菌菌和肠道沙门氏菌的无活化.
- 评估纳秒PEF (nsPEF) 和H2O2对细菌减少的联合作用.
主要方法:
- 细菌用nsPEF (600ns脉冲,21kV/cm,10Hz) 和H2O2的亚抑制度 (0.1-0.5%) 进行了治疗.
- 使用单剂量和分剂量PEF暴露,接触时间从5到45分钟.
- 对大肠杆菌,L. innocua和S. enterica. 的CFU/mL日志减值进行了测量.
主要成果:
- 对于大肠杆菌,通过0.1%的H2O2和15分钟的接触时间,实现了超过5logCFU/mL的减少.
- 在0.5%的H2O2和30分钟的接触时间下,L. innocua和S. enterica的CFU/mL减少达到了>5 log.
- 联合治疗明显比单独治疗更有效,分剂量PEF显示增强的致死性.
结论:
- nsPEF和H2O2的组合提供了一种强大的非热方法来对食品进行脱污.
- 在H2O2暴露后分剂量PEF治疗会增加细菌的易感性.
- 这种协同方法可以实现对食品安全至关重要的显著微生物减少.
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