爆发性病原体效应器AVR-Pik结合并稳定大米重金属相关蛋白 (HMA),以选择它们在免疫中的功能
Kaori Oikawa1, Koki Fujisaki1, Motoki Shimizu1
1Iwate Biotechnology Research Center, Kitakami, Iwate, Japan.
PLoS pathogens
|November 18, 2024
概括
爆发性真菌效应器AVR-Pik针对大米重金属相关 (HMA) 域蛋白,如OsHIPP20,以抑制免疫力. 然而,这种相互作用意外地触发了具有特定NLR受体的植物的免疫力.
科学领域:
- 植物免疫力 植物免疫力
- 分子植物病原体相互作用
- 菌效应剂是一种真菌效应剂.
背景情况:
- 细胞内核酸结合域和富含白素的重复含有 (NLR) 受体对免疫至关重要.
- 植物NLRs通常具有由病原体效应因子目标演变的集成域.
- 类似于这些集成域的蛋白质的功能以及它们被效应器向的功能是不太了解的.
研究的目的:
- 研究爆发性真菌效应剂AVR-Pik与含有重金属相关 (HMA) 域的米蛋白之间的相互作用.
- 确定HMA域蛋白在植物敏感性和免疫力中的作用.
- 阐明病原体作用因子针对宿主蛋白质的进化策略.
主要方法:
- 蛋白结合试验检测AVR-Pik与大米HMA蛋白的相互作用.
- 基因淘汰研究,以评估OsHIPP20在疾病耐药性的功能.
- 在效应体识别时分析疾病症状和植物免疫力.
主要成果:
- 爆炸真菌效应器AVR-Pik结合了大米HMA域蛋白,特别是OsHIPP19和OsHIPP20.
- 通过AVR-Pik结合,可以稳定OsHIPP19和OsHIPP20.
- OsHIPP20的淘汰会增强对爆发病原体的抵抗力,将其识别为易感性基因.
- 在植物中,AVR-Pik与HMA域的结合触发了在它们的NLR受体中集成HMA域的免疫力.
结论:
- 爆发性真菌效应体AVR-Pik已经进化到结合和选择米HMA域蛋白,可能抑制宿主免疫力.
- OsHIPP20作为一种易感基因,促进疾病的发展.
- 虽然AVR-Pik结合抑制了基底免疫力,但矛盾的是,它激活了含有特定NLR-HMA受体的植物的防御,揭示了进化的权衡.
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