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相关概念视频

Protein Glycosylation01:25

Protein Glycosylation

6.8K
Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
6.8K
Oligosaccharide Assembly01:24

Oligosaccharide Assembly

2.8K
Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...
2.8K
Proteoglycans01:05

Proteoglycans

3.9K
Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
3.9K
Glycocalyx and its Functions01:14

Glycocalyx and its Functions

3.7K
The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
3.7K
Glycosaminoglycans01:23

Glycosaminoglycans

4.7K
Glycosaminoglycans (GAGs), also known as mucopolysaccharides, are long and linear polymers comprising of specific repeating disaccharides - the amino sugar that can be N-acetylglucosamine or N-acetylgalactosamine, and a uronic acid that is usually glucuronic acid or iduronic acid.
GAGS are found in the extracellular matrix of vertebrates, invertebrates, and bacteria. Due to their polar nature they attract water, and serve as excellent lubricants or shock absorbers in an animal body.
Hyaluronic...
4.7K
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

3.8K
Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
3.8K

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相关实验视频

Updated: Jun 7, 2025

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
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Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

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IgG 糖化:生物标记物,功能调节器和结构组件.

Barbara Radovani1,2, Falk Nimmerjahn2,3

  • 1Faculty of Biotechnology and Drug Development, University of Rijeka, Rijeka, Croatia.

Journal of immunology (Baltimore, Md. : 1950)
|November 18, 2024
PubMed
概括

免疫球蛋白G (IgG) 糖化对适应性免疫至关重要,影响结构和功能. 了解IgG糖型是治疗开发和优化免疫反应的关键.

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Glycan Node Analysis: A Bottom-up Approach to Glycomics
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Glycan Node Analysis: A Bottom-up Approach to Glycomics

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Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
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Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases

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相关实验视频

Last Updated: Jun 7, 2025

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples
13:21

Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

Published on: May 4, 2012

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Glycan Node Analysis: A Bottom-up Approach to Glycomics
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Glycan Node Analysis: A Bottom-up Approach to Glycomics

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Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases
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Identification and Characterization of Protein Glycosylation using Specific Endo- and Exoglycosidases

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科学领域:

  • 免疫学 免疫学 免疫学
  • 葡萄糖生物学 葡萄糖生物学
  • 生物化学 生物化学

背景情况:

  • 免疫球蛋白G (IgG) 抗体是适应性免疫的核心.
  • 对IgG的糖化是维持其结构完整性和调节效应器功能的必要条件.

研究的目的:

  • 审查IgG糖化处理的细胞生物学,治疗和与疾病有关的方面.
  • 探索IgG糖化对疾病调节和治疗结果的影响.
  • 讨论开发诱导Ag特定IgG的疫苗的潜力.

主要方法:

  • 关于IgG糖化酶的现有文献的综述.
  • 在不同IgG区域 (Fab和Fc) 中分析糖结构.
  • 检查IgG糖化酶的功能后果.

主要成果:

  • 在不同的区域 (Fab与Fc) 中,IgG糖化模式有所不同.
  • 糖基化显著影响IgG在疾病调节中的作用.
  • 特定的IgG糖型会影响治疗疗效和免疫反应.

结论:

  • 研究IgG糖化对理解其动态和功能影响至关重要.
  • 对IgG糖化酶的洞察力可以促进未来的研究和临床应用.
  • 向IgG糖化定位为新的治疗策略和疫苗设计提供了潜力.