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在树突细胞上表达Toxoplasma GRA15,抑制B细胞分化和抗体产生

Yuki Nakayama¹, Fumiaki Ihara¹, Daisuke Okuzaki²

¹Department of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka 565-0871, Japan; Laboratory of Immunoparasitology, WPI Immunology Frontier Research Center, Osaka University, Suita, Osaka 565-0871, Japan.

Parasitology international

|November 18, 2024

概括

这种毒性淋巴细胞 (Toxoplasma gondii) 是

科学领域:

免疫学免疫学免疫学
寄生虫学的寄生虫学
分子生物学分子生物学

背景情况:

托克索普拉斯马淋巴菌 (T. gondii) 密集颗粒蛋白15 (GRA15) 通过NF-κB激活天生的免疫力.
GRA15在寄生虫优势和宿主-寄生虫相互作用中的作用在很大程度上是未知的.

研究的目的:

为了研究GRA15在宿主免疫抑制中的作用,T. gondii.
为了识别由GRA15针对免疫逃避的宿主细胞类型.

主要方法:

在C57BL/6小鼠中使用野生型和Δgra15 T. gondii的比较感染研究.
条件敲门小鼠 (CD11c-Cre/GRA15cKI) 的生成以在特定细胞系中表达GRA15.
评估感染或免疫接种后的抗体标位和免疫细胞群.

主要成果:

与 Δgra15 感染相比,T. gondii 感染野生型寄生虫导致抗T. gondii 抗体标位和血细胞数量减少.
树突细胞中的GRA15表达,但不是巨细胞,抑制了T. gondii抗体的产生.
树突细胞中的GRA15降低了抗卵胺抗体标位,并在免疫小鼠中减少了OVA特异性T细胞数量.

结论:

由T. gondii分泌的GRA15抑制宿主获得的免疫力,特别是T细胞介导的幽默免疫力.
树突细胞是GRA15介导免疫抑制的关键目标.
了解GRA15的作用可能有助于开发Toxoplasma疫苗.

关键词:

B细胞是B细胞的组成部分.在GRA15中使用GRA15.血细胞和生殖中心 B 细胞.产生特定抗体的生产.这种毒性淋巴细胞 (Toxoplasma gondii) 是

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