相关实验视频

Updated: Jun 7, 2025

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Next Generation Sequencing for the Detection of Actionable Mutations in Solid and Liquid Tumors

Published on: September 20, 2016

分子病理学的下一步:细胞学中的下一代测序

Ricella Souza da Silva^1,2, Fernando Schmitt^1,2,3

¹IPATIMUP Diagnostics, IPATIMUP - Institute of Molecular Pathology and Immunology of Porto University, Porto, Portugal.

Journal of pathology and translational medicine

|November 18, 2024

概括

精密瘤学现在在治疗决策中使用分子分析,下一代测序 (NGS) 已成为更广泛的癌症测试标准. 优化细胞学样本的NGS增强分子分析和向治疗选择.

科学领域:

在瘤学瘤学.
分子生物学分子生物学
遗传学是一个遗传学.

背景情况:

精确瘤学正在从形态诊断转向治疗决策的分子分析.
下一代测序 (NGS) 越来越多地被推用于更广泛的癌症检测,因为它的效率和临床价值.
细胞学样本为获得用于分子分析的遗传物质提供了一种不那么侵入性的方法.

研究的目的:

优化细胞学样本在下一代测序 (NGS) 中用于分子分析的使用.
突出利用细胞学样本在确定可操作的分子变化的好处,在固体瘤的向治疗.
讨论将细胞学和液体活检与组织活检相结合的策略,以进行全面的瘤基因定型.

主要方法:

对瘤学中NGS的当前指导方针和文献的审查.
分析细胞学样本对分子分析的适用性.
探索在临床实践中组合不同样本类型 (细胞学,液体,组织) 的策略.

主要成果:

细胞学样本可以产生高质量的遗传物质,用于敏感和高效的NGS分析.
优化细胞学样本的使用可以帮助识别针对性治疗的可操作分子变化.
整合各种样本类型为瘤基因类型和监测提供了一个全面的方法.

结论:

细胞学样本的下一代测序 (NGS) 是精密瘤学的宝贵工具.
结合细胞学,液体和组织活检,为生物标志物表征提供了一个全面的策略.
需要进一步的验证研究才能将这些方法完全整合到常规临床实践中.

关键词:

身体液体身体液体细胞学细胞学分子病理学分子病理学下一代测序测序是什么

更多相关视频

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer

Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer

Published on: September 8, 2023

相关概念视频

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

Karyotyping

Karyotyping

Sanger Sequencing

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...