乳腺癌组织中基本元素和非基本金属的定量分布由LA-ICP-TOF-MS
Sara Escudero-Cernuda1, David Clases2, Noemi Eiro3
1Department of Physical and Analytical Chemistry, University of Oviedo, Oviedo, Spain.
Analytical and bioanalytical chemistry
|November 18, 2024
概括
铁,铜,,和等基本和非基本元素在乳腺瘤中升高. 它们在瘤中的明显分布有助于区分乳腺癌组织和进展.
科学领域:
- 生物医学科学 生物医学科学
- 分析化学是一种分析化学.
- 在瘤学瘤学.
背景情况:
- 乳腺癌 (BC) 是全球女性癌症死亡的主要原因.
- 识别新的生物标志物对于改善BC诊断,预防和预后至关重要.
- 基本的微量金属 (Fe,Cu,Zn) 和非基本元素可能会影响BC病理生理学和进展.
研究的目的:
- 研究乳腺组织中基本和非基本元素的空间分布和度.
- 评估元素分析对乳腺癌诊断应用的潜力.
- 为了比较转移性,非转移性和健康乳腺组织中的元素概况.
主要方法:
- 使用激光切除 (LA) 与感应合的等离子体飞行时间质谱法 (ICP-ToF-MS) 相结合.
- 使用基于凝的标准进行了量化,用于外部校准.
- 在转移性,非转移性和健康的乳腺组织中绘制了元素分布图.
主要成果:
- 与健康组织相比,瘤样本中的Fe,Cu,Zn,Sr和Ba水平显著增加.
- Sr和Ba显示出强烈的正相关性,表明类似的化学行为.
- 元素测绘区分了组织类型 (瘤与肌层),并揭示了瘤中的较高元素水平.
- 与非转移性瘤相比,转移性瘤在瘤中显示出明显更高的Fe积累.
结论:
- 使用LA-ICP-ToF-MS的元素分析可以区分健康,非转移和转移的乳腺组织.
- 特定的元素,特别是Fe,显示与BC进展相关的差异积累模式.
- 这种技术有望开发乳腺癌的新诊断和预后工具.
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