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Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Updated: Jun 17, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

一个模块化平台用于生物发光RNA跟踪.

Lila P Halbers¹, Kyle H Cole², Kevin K Ng¹

¹Department of Pharmaceutical Sciences, University of California, Irvine, Irvine, CA, USA.

Nature communications

|November 18, 2024

概括

此摘要是机器生成的。

研究人员开发了一个新的生物发光RNA成像平台. 这种方法能够在细胞和活体动物中检测出敏感的,单复制的RNA,克服了光探针的局限性.

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Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder ALLIGATOR

Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder ALLIGATOR

Published on: December 13, 2017

相关实验视频

Last Updated: Jun 17, 2026

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters

Published on: September 27, 2012

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder ALLIGATOR

Flexible Measurement of Bioluminescent Reporters Using an Automated Longitudinal Luciferase Imaging Gas- and Temperature-optimized Recorder ALLIGATOR

Published on: December 13, 2017

科学领域:

分子生物学分子生物学
生物技术是生物技术.
生物物理学的生物物理.

背景情况:

了解RNA生物学需要在体内进行转录跟踪.
目前的化探测器在灵敏度,动态范围和深度方面面临限制,原因是激发光和自光.

研究的目的:

开发一种新的生物发光平台,用于在体内进行序列RNA成像.
克服现有的光RNA检测方法的局限性.

主要方法:

工程RNA标签在转录时招募 luciferase 片段 (RNA 灯).
利用生物发光来产生光子和对RNA点进行成像.
在细胞和活体动物模型中都已经证明了检测.

主要成果:

从细胞和活体动物中的RNA点中实现了强大的光子产生.
启用敏感检测,只有一个单一副本的RNA标签.
与多副本光平台相比,显示出显著的改进.

结论:

开发的生物发光平台为RNA可视化提供了基础工具.
这项技术允许在不同尺度上对RNA动态进行敏感的串行成像.
在生物研究中为体内RNA跟踪提供了一个有前途的替代方案.