全长度直接RNA测序揭示了RNA表达,处理和修改在衰老中的广泛重塑
Erin C Schiksnis1, Ian A Nicastro1, Amy E Pasquinelli1
1Department of Molecular Biology, School of Biological Sciences, 9500 Gilman Drive, University of California, San Diego, La Jolla, CA 92093-0349, USA.
Nucleic acids research
|November 18, 2024
概括
这项研究揭示了新型RNA异型和RNA处理在C. elegans的衰老过程中下降. 伪尿素修饰随着年龄的增长而增加,为衰老机制提供了新的见解.
科学领域:
- 分子生物学分子生物学
- 基因组学就是基因组学.
- 衰老研究研究 衰老研究
背景情况:
- 生物的衰老的特点是细胞功能障碍和解剖学衰退.
- 了解衰老的分子机制对于开发干预措施至关重要.
- RNA测序提供了对基因表达和调节的见解.
研究的目的:
- 为了研究Caenorhabditis elegans的转录组中与年龄相关的变化.
- 使用长读序列识别识别新型RNA异型和修饰.
- 绘制RNA编辑部位的地图,包括伪尿素修饰,以及它们的年龄相关变化.
主要方法:
- 进行了标准RNA测序 (RNA-seq) 和牛津纳米孔技术直接RNA-seq.
- 在Caenorhabditis elegans的成年时间过程中进行了分析.
- 进行了RNA处理忠实度和RNA编辑的全基因组分析.
主要成果:
- 确定了数百种新的RNA异型和与年龄相关的差异异异型积累.
- 在整个基因组中观察到RNA处理忠实度的下降.
- 检测到数千个inosin和数百个伪乌里丁编辑,伪乌里丁修改随着年龄的增长而增加.
结论:
- 这项研究确定了与C. elegans.老龄化相关的转录组签名.
- 长读测序揭示了在衰老过程中对替代拼接和RNA处理的新见解.
- 这项工作为了解与年龄相关的基因表达和转录后调节提供了宝贵的资源.
相关概念视频
01:23Types of RNA
Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
01:53RNA Stability
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
03:21RNA-seq
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...
01:20Types of RNA
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
RNA Performs Diverse...
02:51mRNA Stability and Gene Expression
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability