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用基和过氧化酶进行序列预处理,以有效的酶式糖化玉米炉的玉米炉
Man Zhou1, Yaru Wang1, Yuan Wang1
1State Key Laboratory of Animal Nutrition and Feeding, Institute of Animal Science, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Biotechnology for biofuels and bioproducts
|November 19, 2024
概括
白色腐烂真菌使用基 (⋅OH) 和过氧化酶 (MnPs) 顺序分解玉米. 这种联合预处理显著提高了酶的糖化和纤维素的可访问性.
科学领域:
- 生物技术是生物技术.
- 生物化学 生化学
- 菌类学 菌类学是指菌类学.
背景情况:
- 白色腐烂真菌产生反应性氧物种和酶,用于分解纤维素.
- 这些药物的特殊作用和相互作用在克服糖化阻碍的纤维素干细胞阻碍物方面尚不完全理解.
研究的目的:
- 为了研究从Irpex lacteus中基 (⋅OH) 和过氧化酶 (MnPs) 的连续作用.
- 评估⋅OH和MnP预处理对玉米炉的协同效应,以提高酶糖化.
主要方法:
- 机密基因分析和细胞外酶活性测定Irpex乳腺菌.
- 在玉米炉上对⋅OH和纯化的重组MnPs进行了体外功能研究.
- 在序列式⋅OH和MnP预处理后,对酶糖化和纤维素可访问性的评估.
主要成果:
- 机密原子分析显示,在纤维素分解过程中,⋅OH和MnPs的序列表达.
- ⋅OH破坏了玉米炉的表面结构,增加了糖化和纤维素的可访问性.
- MnPs分裂了素模型二次体,顺序的⋅OH和MnP预处理显示出协同效应,促进了糖化 (2.9倍) 和纤维素可访问性 (1.8倍).
结论:
- 首次证明了连续的⋅OH和MnP预处理对玉米炉的协同效应.
- 突出了⋅OH和MnP预处理对玉米炉的高效酶糖化潜力.
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