绿色合成的纳米颗粒利用Drimia indica:洞察到抗癌和抗菌活动
Fuad Ameen1, Norah Salem Almalki2, Rawan Alshalan1
1Department of Botany & Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia.
Microscopy research and technique
|November 19, 2024
概括
纳米颗粒 (DI-SeNPs) 通过诱导亡,对肺癌细胞产生强大的抗癌作用. 这些纳米颗粒还显示出针对各种病原体的显著抗微生物特性.
科学领域:
- 纳米技术 纳米技术
- 材料科学 材料科学 材料科学
- 生物医学工程 生物医学工程
背景情况:
- 纳米粒子 (SeNPs) 已因其治疗潜力而得到认可.
- 药用植物为纳米材料合成提供可持续的来源.
- indica是一种具有传统药用历史的植物.
研究的目的:
- 通过使用Drimia indica叶提取物 (DI-SeNPs) 合成和表征纳米颗粒.
- 评估DI-SeNPs对人类肺腺癌细胞的抗癌活性 (A549).
- 评估DI-SeNPs对细菌和真菌的抗菌疗效.
主要方法:
- 使用Drimia indica树叶水性提取物 (DI-LAE) 合成SeNP.
- 使用UV-Vis,TEM,EDX,XRD,泽塔电位和FTIR进行表征.
- 在A549细胞中的细胞毒性,ROS生成,细胞循环停止,DNA损伤和细胞亡的体外评估.
- 对特定细菌和真菌菌株的抗微生物活性评估.
主要成果:
- DI-SeNPs已经成功合成和表征.
- DI-SeNPs对A549细胞表现出剂量依赖的细胞毒性 (IC50 = 43.21μg/mL).
- 作用机制包括ROS增加,G2/M细胞循环停止,DNA损伤,亡诱导 (通过p53,Akt和caspase 3调制) 和广泛的抗菌活性.
结论:
- 来自Drimia indica的DI-SeNPs具有显著的抗癌和抗菌潜力.
- 抗癌效应是由ROS生成,细胞循环停止和细胞亡介导的.
- DI-SeNPs代表了一种有前途的纳米材料,用于癌症和传染病的治疗应用.
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