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Updated: Jun 7, 2025

Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
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Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations

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开发一种新的基因型与表型相关的抗体查系统

Takashi Watanabe1, Hikaru Hata2, Yoshiki Mochizuki1

  • 1Laboratory for Integrative Genomics, RIKEN Center for Integrative Medical Sciences, Yokohama, Japan.

eLife
|November 19, 2024
PubMed
概括

我们开发了一种快速查重组单克隆抗体的方法,使用一种新的双表达向量系统. 这种技术可以在七天内有效地分离高亲和抗体,用于治疗和诊断应用.

关键词:
黄金之门 克隆 复制免疫学 免疫学 免疫学这是一种炎症炎症炎症炎症.流感是广泛反应性的流感.膜结合的Ig表达方式这里是鼠标鼠标鼠标鼠标鼠标鼠标.基于ngs的Ig-seqq.

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Hybridoma Technology01:31

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
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Commonly used fusion techniques — electroporation,...
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