Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K
Channel Rhodopsins01:11

Channel Rhodopsins

2.5K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
2.5K
Reporter Genes02:11

Reporter Genes

11.2K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Dynamic heterogeneity in an E. coli stress response regulon mediates gene activation and antimicrobial peptide tolerance.

Cell reports·2026
Same author

Single-cell characterization of bacterial optogenetic Cre recombinases.

iScience·2026
Same author

Single-cell characterization of bacterial optogenetic Cre recombinases.

bioRxiv : the preprint server for biology·2025
Same author

Unique growth and morphology properties of Clade 5 <i>Clostridioides difficile</i> strains revealed by single-cell time-lapse microscopy.

bioRxiv : the preprint server for biology·2025
Same author

Evaluating the predictive power of combined gene expression dynamics from single cells on antibiotic survival.

mSystems·2025
Same author

Label-free nanoscopy of cell metabolism by ultrasensitive reweighted visible stimulated Raman scattering.

Nature methods·2025

相关实验视频

Updated: Jun 7, 2025

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

4.5K

红光响应重组酶用于细菌光遗传学

Fereshteh Jafarbeglou1,2, Mary J Dunlop1,2

  • 1Biomedical Engineering, Boston University, Boston, Massachusetts 02215, United States.

ACS synthetic biology
|November 19, 2024
PubMed
概括

我们开发了OptoCre-REDMAP,这是一个红光激活系统,用于微生物工程中的精确控制. 这种工具可以实现先进的合成生物学应用,改善光线透率和对蓝光系统的直角性.

科学领域:

  • 合成生物学 合成生物学
  • 微生物工程 微生物工程
  • 视觉遗传学 视觉遗传学

背景情况:

  • 光遗传工具在微生物工程中提供可调节的控制.
  • 目前的系统主要使用蓝光,限制了多色应用.
  • 红灯提供了诸如更深的透和减少毒性等好处.

研究的目的:

  • 介绍OptoCre-REDMAP,一种红光诱导的Cre复合酶系统,用于*Escherichia coli*.
  • 通过使用红光,能够精确控制基因表达和DNA切除.
  • 在微生物合成生物学中推进多色控制.

主要方法:

  • 它利用了植物光受体 (PhyA,FHY1) 和分裂的Cre复合酶.
  • 优化了Cre启动密码子和特征感应水平.
  • 对光强度,持续时间和环境条件的测试灵敏度.

主要成果:

  • 通过红光实现最小的黑暗表达和快速激活 (4h内).
  • 在各种条件下证明了OptoCre-REDMAP的可靠性和灵活性.
  • 展示了与蓝光系统 (OptoCre-VVD) 和优越的红光透的直角控制.
关键词:
克雷复合酶可以重组.细菌光遗传学 细菌光遗传学多色调控制的多色调控制红灯是红色的,红灯是红色的.

更多相关视频

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.3K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

504

相关实验视频

Last Updated: Jun 7, 2025

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo
07:19

Bioluminescent Optogenetics 2.0: Harnessing Bioluminescence to Activate Photosensory Proteins In Vitro and In Vivo

Published on: August 4, 2021

4.5K
Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.3K
Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation
08:00

Spatiotemporal Control of Protein Activity through Optogenetic Allosteric Regulation

Published on: October 4, 2024

504

结论:

  • OptoCre-REDMAP为微生物应用提供了强大的,选择性的红光控制.
  • 该系统适用于需要精确多色控制的先进合成生物学.
  • 对于特定的微生物工程任务,红光比蓝光有优势.