富含线粒体的微囊泡通过转移线粒体和抑制局部铁亡来缓解CNI ED
Zhenkang Liang1, Zehong Chen1, Chaowei Zhang1
1Department of Gastrointestinal Surgery, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510630, People's Republic of China.
International journal of nanomedicine
|November 19, 2024
概括
富含线粒体的微粒细胞 (MVs) 有效地治疗由洞穴神经损伤 (CNI) 引起的勃起功能障碍 (ED). 通过转移线粒体,MVs恢复勃起功能,为CNI诱导的ED提供了一种新的治疗方法.
科学领域:
- 再生医学是一种再生医学.
- 细胞生物学 细胞生物学
- 泌尿器科 泌尿器科 泌尿器科 泌尿器科
背景情况:
- 勃起功能障碍 (ED) 是像直肠和前列腺手术一样的盆腔手术后的常见并发症.
- 洞穴神经损伤 (CNI) 后的ED目前的治疗方法缺乏最终的解决方案.
- 了解CNI诱导ED背后的机制对于开发有效疗法至关重要.
研究的目的:
- 为了研究富含线粒体的微 (MVs) 治疗因CNI而产生的ED的治疗潜力.
- 阐明MVs在CNI引起的ED中发挥其治疗作用的潜在机制.
主要方法:
- 从PC12细胞中分离出MV和线粒体 (MT).
- 试验室研究评估了MV和MT对体光滑肌细胞 (CCSMC) 的影响,评估了细胞亡,线粒体膜潜力 (MMP),活性氧物种 (ROS),线粒体ROS (mtROS),铁,甲基 (MDA) 和抗氧化活性.
- 在体内研究中,使用PBS,MV或MT治疗的CNI诱导的ED大鼠模型,随后进行勃起功能评估和组织学分析. 机械学研究涉及抑制MVs内的线粒体.
主要成果:
- 在实验室中对CCSMC的MV治疗显著降低了细胞亡,ROS,mtROS,铁和MDA水平,同时增加了MMP和内生抗氧化系统活性.
- 在体内,MVs移植显著恢复了CNI-ED大鼠的勃起功能和光滑肌肉含量.
- 救援实验表明,MV转移线粒体以发挥其治疗作用.
结论:
- MVs移植是对CNI诱导的ED有前途的治疗方法.
- MVs可能通过线粒体转移在受伤部位对抗氧化应激和铁亡.
- MVs代表了CNI诱导ED的潜在治疗工具.
相关概念视频
01:19Translocation of Proteins into the Mitochondria
3.0K
Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
3.0K
01:46Electron Transport Chain: Complex I and II
11.9K
The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
ROS generation is regulated and maintained at moderate levels necessary...
ROS generation is regulated and maintained at moderate levels necessary...
11.9K
01:39Mitochondrial Protein Sorting
4.3K
Mitochondria are double-membrane organelles of the eukaryotes involved in cellular metabolism, signaling, ATP synthesis, and programmed cell death. Each of these processes requires specific proteins and enzymes that must be correctly sorted to the right mitochondrial subcompartment for the proper functioning of the organelle.
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
Most of these mitochondrial proteins are encoded by the nucleus and imported to the mitochondria as unfolded or loosely folded precursors. Mitochondrial precursors...
4.3K
01:45Vesicular Tubular Clusters
2.4K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.4K