口腔状细胞癌,明显正常的相邻粘膜和上皮质失生症的基因组学分析
Majid Mirhashemi1, Nasrollah Saghravanian1, Narges Ghazi1
1Department of Oral and Maxillofacial Pathology, School of Dentistry, Mashhad University of Medical Sciences, Mashhad, Iran.
概括
口腔状细胞癌 (OSCC) 和其前体病变的基因组学变化揭示了核与细胞质比率的显著增加. 这些细胞变化可以预测前恶性口腔组织中的恶性转变潜力.
科学领域:
- 口腔病理学 口腔病理学
- 癌症研究 癌症研究
- 历史形态测量 历史形态测量 历史形态测量
背景情况:
- 口腔状细胞癌 (OSCC) 是最常见的口腔癌,与前恶性病变相比,对细胞变化的研究有限.
- 组织形态测量,细胞和组织结构的研究,为OSCC发展提供了潜在的见解.
研究的目的:
- 为了评估和比较OSCC的细胞基因组学变化,失塑性白血病 (DL),非失塑性白血病 (NDL) 和正常的口腔粘膜 (NOM).
- 为了确定标志着恶性转变潜力的组织形态测量参数.
主要方法:
- 一项横截面研究分析了来自六组的72个样本:OSCC表面 (SCCSF),OSCC侵入性前部 (SCCIF),明显正常的邻近口腔粘膜 (SCCANM),DL,NDL和NOM (每组12个样本).
- 使用ANOVA.H&E分析了染色的血素和欧 (H&E) 样本,以核与细胞质比率 (N/C),核面积 (NA) 和细胞面积 (CA) 进行分析.
- 一个P值<0.05被认为是显著的.
主要成果:
- 观察到CA,NA和N/C的显著增加在从正常上皮层到异形上皮层和OSCC的基底和准基底层.
- 在SCCIF和SCCSF组中发现了最高的NA,CA和N/C,而NOM显示了最低的值.
- 与NOM相比,SCCANM基底和基准细胞呈现出显著的N/C差异,这表明恶性转变风险很高.
结论:
- 细胞组织形态测量从正常组织经历明显的变化,通过前恶性病变到OSCC.
- 像N/C,NA和CA这样的参数可以作为预测前恶性口腔病变中恶性转变潜力的有价值指标.
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