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在非小细胞肺癌患者中,miR-10a-3p的预后值

Julija Simiene^1,2, Linas Kunigenas^1,2, Rimvile Prokarenkaite^1,2

¹Laboratory of Molecular Oncology, National Cancer Institute, Vilnius, LT-08406, Lithuania.

OncoTargets and therapy

|November 19, 2024

概括

这项研究确定了血miR-10a-3p作为非小细胞肺癌 (NSCLC) 进展的有希望的非侵入性生物标志物. 增加的miR-10a-3p水平与NSCLC患者的延长无进展生存时间相关.

科学领域:

在瘤学瘤学.
生物标志物发现发现
分子生物学分子生物学

背景情况:

非小细胞肺癌 (NSCLC) 患者的结局需要新的生物标志物来早期和不那么侵入性地检测.
微RNA (miRNA) 表达正在被探索为各种癌症的潜在诊断和预后工具.

研究的目的:

研究miRNA表达作为非小细胞肺癌 (NSCLC) 进展的生物标志物的潜力.
在3D细胞培养系统中培养的NSCLC细胞中选miRNA.

主要方法:

具有不同攻击性的NSCLC细胞系和非癌性支气管上皮细胞系在3D培养.
使用Illumina NGS.小RNA库被准备和测序使用Illumina NGS.
在89个配对的NSCLC组织和血样本中验证了差异表达的miRNAs.

主要成果:

下一代测序确定了NSCLC细胞系中8种差异表达的miRNA.
血miR-10a-3p显著增加,并与延长无进展生存期 (PFS) 相关 (p=0.009).
血miR-10a-3p是NSCLCPFS的一个显著的预后变量 (HR:0.5,p=0.029).

结论:

在3DNSCLC细胞培养中查miRNA表达,确定了潜在的生物标志物.
循环中的miR-10a-3p显示出作为一种新型,非侵入性生物标志物的潜力,用于NSCLC患者的短期预后.

关键词:

三维细胞培养的3D细胞培养在NSCLCLC中,我们可以看到.这就是miRNAs.非侵入性的临床生物标志物幸存率幸存率生存率

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