Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Calmodulin-dependent Signaling01:16

Calmodulin-dependent Signaling

5.1K
Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...
5.1K
Cross-bridge Cycle01:26

Cross-bridge Cycle

116.9K
As muscle contracts, the overlap between the thin and thick filaments increases, decreasing the length of the sarcomere—the contractile unit of the muscle—using energy in the form of ATP. At the molecular level, this is a cyclic, multistep process that involves binding and hydrolysis of ATP, and movement of actin by myosin.
116.9K
Muscle Contraction01:10

Muscle Contraction

6.2K
In skeletal muscles, acetylcholine is released by nerve terminals at the motor endplate—the point of synaptic communication between motor neurons and muscle fibers. The binding of acetylcholine to its receptors on the sarcolemma allows entry of sodium ions into the cell and triggers an action potential in the muscle cell. Thus, electrical signals from the brain are transmitted to the muscle. Subsequently, the enzyme acetylcholinesterase breaks down acetylcholine to prevent excessive...
6.2K
Smooth Muscle Contraction01:25

Smooth Muscle Contraction

2.5K
Smooth muscle contraction is a complex process vital for various bodily functions, from maintaining blood vessel tension to facilitating the movement of food through the digestive tract. Unlike striated muscles, smooth muscle contraction begins more slowly and lasts longer.
The onset of contraction is triggered by an increase in calcium ions within the sarcoplasm, similar to the process in striated muscle. However, smooth muscles have a relatively smaller reservoir of the sarcoplasmic...
2.5K
Relaxation of Skeletal Muscles01:29

Relaxation of Skeletal Muscles

3.0K
The period of muscle contraction primarily influences the duration of stimulation at the neuromuscular junction (NMJ), the presence of free calcium ions in the sarcoplasm, and the availability of energy or ATP to support contractions.
When an action potential reaches the axon terminal, it depolarizes the membrane and opens voltage-gated sodium channels. Sodium ions enter the cell, further depolarizing the presynaptic membrane. This depolarization causes voltage-gated calcium channels to open....
3.0K
Actin and Myosin in Muscle Contraction01:16

Actin and Myosin in Muscle Contraction

8.9K
Actin and myosin are contractile proteins that form the sarcomere found in skeletal muscle tissues for regulating muscle contraction. Actin, a globular contractile protein, interacts with myosin for muscle contraction. The skeletal tissue appears striped or striated under a microscope due to the repeated arrangement of contractile proteins actin and myosin along the length of myofibrils. Dark A bands and light I bands repeat along myofibrils, and the alignment of myofibrils in the cell causes...
8.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Association of athlete's heart and ACE I/D genotype with later-life left ventricular hypertrophy: a retrospective cohort study.

BMC cardiovascular disorders·2026
Same author

An immune-associated mitochondrial DNA variant with sex differences reveals a putative novel microprotein called MASL.

Immunity & ageing : I & A·2026
Same author

Collagen Type V alpha 1 chain and alpha-actinin-3 variants predict knee ligament injury risk in professional football players.

Journal of experimental orthopaedics·2026
Same author

Impact of pre-vaccination active vitamin D levels on COVID-19 mRNA vaccine-induced immunity in a Japanese cohort.

Vaccine·2026
Same author

Association of the ACTN3 Genotype with Muscle Function: A Systematic Review and Meta-analysis.

International journal of sports medicine·2026
Same author

RAB3GAP2 is a regulator of skeletal muscle endothelial cell proliferation and associated with capillary-to-fiber ratio.

Cell reports·2026

相关实验视频

Updated: Jun 7, 2025

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
10:45

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Published on: July 26, 2017

10.0K

MOTS-c通过直接结合和激活CK2来调节骨肌肉功能.

Hiroshi Kumagai1, Su-Jeong Kim1, Brendan Miller1

  • 1The Leonard Davis School of Gerontology, University of Southern California, Los Angeles, CA, USA.

iScience
|November 19, 2024
PubMed
概括

线粒体微蛋白MOTS-c激活蛋白激酶CK2,改善新陈代谢和预防肌肉缩. 这种互动对MOTS-cc至关重要.

关键词:
生理学 生理学 生理学细胞生物学 细胞生物学

更多相关视频

Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations
09:50

Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations

Published on: January 25, 2018

6.7K
Myo-mechanical Analysis of Isolated Skeletal Muscle
08:42

Myo-mechanical Analysis of Isolated Skeletal Muscle

Published on: February 22, 2011

26.8K

相关实验视频

Last Updated: Jun 7, 2025

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement
10:45

Isolation of Human Myoblasts, Assessment of Myogenic Differentiation, and Store-operated Calcium Entry Measurement

Published on: July 26, 2017

10.0K
Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations
09:50

Application of Chronic Stimulation to Study Contractile Activity-induced Rat Skeletal Muscle Phenotypic Adaptations

Published on: January 25, 2018

6.7K
Myo-mechanical Analysis of Isolated Skeletal Muscle
08:42

Myo-mechanical Analysis of Isolated Skeletal Muscle

Published on: February 22, 2011

26.8K

科学领域:

  • 线粒体生物学 线粒体生物学
  • 代谢调节 代谢调节 代谢调节
  • 老龄化的分子机制.

背景情况:

  • 12S rRNA-c (MOTS-c) 的线粒体开放读取框架是一种调节新陈代谢并对抗与年龄相关的疾病的类激素.
  • 确切的分子标和MOTS-c有益作用的基础机制仍然不完全理解.

研究的目的:

  • 识别和功能性地描述MOTS-c.的直接分子标.
  • 阐明这些目标在调解MOTS-c对新陈代谢和肌肉健康的生理影响中的作用.

主要方法:

  • 在体外结合测试以评估MOTS-c与候选蛋白的相互作用.
  • 无细胞激酶测试以确定MOTS-c对蛋白激酶活性的影响.
  • 使用小鼠模型进行体内研究,以评估MOTS-c给药和CK2抑制对骨肌肉和葡萄糖代谢的影响.
  • 对脂肪和肌肉组织的组织特异性影响和分子相互作用的分析.
  • 研究一种自然存在的MOTS-c变体 (K14Q MOTS-c) 和其功能后果.

主要成果:

  • MOTS-c直接与蛋白激酶CK2 (素激酶2) 结合并激活.
  • 在小鼠中使用MOTS-c可以预防骨肌肉缩和增强葡萄糖吸收,其效果取决于CK2活性.
  • MOTS-c表现出组织特异性作用,激活肌肉中的CK2,但抑制脂肪中的CK2.
  • 一种自然存在的变体,K14Q MOTS-c,显示了减少的CK2结合,缺乏MOTS-c的生理效应.
  • 携带K14Q MOTS-c的男性携带者患上了缩症和2型糖尿病 (T2D) 的风险增加,而女性则表现出特定于年龄的T2D风险降低.

结论:

  • 蛋白激酶CK2是MOTS-c.的代谢和抗缩作用所需的直接和功能性标.
  • 在不同的组织中,MOTS-c对CK2的不同调节有助于其组织特异性的作用.
  • 自然发生的MOTS-c变体与受损的CK2相互作用可能会影响对代谢障碍和肉症的易感性.