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Imaging Biological Samples with Optical Microscopy

Imaging Biological Samples with Optical Microscopy

Optical microscopy uses optic principles to provide detailed images of samples. Antonie van Leeuwenhoek designed the first compound optical microscope in the 17th century to visualize blood cells, bacteria, and yeast cells. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes with enhanced magnification and resolution.
In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...

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Updated: Jun 29, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

企:一个快速高效的图像预处理工具,用于多重空间蛋白质组学.

A M Sequeira^1,2, M E Ijsselsteijn², M Rocha¹

¹Department of Informatics, School of Engineering, University of Minho, Braga, Portugal.

Computational and structural biotechnology journal

|November 19, 2024

概括

此摘要是机器生成的。

我们开发了PENGUIN,这是一个易于使用的工具,用于消除多重空间蛋白质组学成像数据. 这种方法可以增强下游分析,例如在没有手动输入的情况下进行细胞细分.

关键词:

背景减去减去背景减去拒绝这种行为,就是拒绝.免疫型定型免疫型定型多重复合成像系统的成像.规范化规范化规范化空间奥米克斯空间奥米克斯

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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

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Last Updated: Jun 29, 2026

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Published on: April 8, 2016

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

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Published on: July 6, 2022

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples

Published on: April 7, 2023

科学领域:

空间生物学空间生物学
蛋白质组学是指蛋白质组学.
生物信息学是一种生物信息学.

背景情况:

多复合空间蛋白质组学为生物系统提供了洞察力.
分析成像数据在降低噪声和正常化方面面临挑战.
现有的消除噪音的解决方案往往是复杂的或需要人工干预.

研究的目的:

介绍PENGUIN (百分比规范化GUI图像deNoising),一个新的图像预处理工具.
为了提供一个用户友好的,有效的解决方案,以消除多重空间蛋白质组学数据.
为了提高下游分析的准确性,如细胞细分和表型.

主要方法:

企利用百分位标准化来调整信号与噪声比.
该工具在没有手动注释或机器学习模型的情况下运行.
它以脚本和Jupyter笔记本为灵活的参数调整提供.

主要成果:

企有效地减少背景噪声,同时保持信号强度.
该工具在细胞细分和表型化任务中表现出更好的性能.
对比分析显示,Penguin的性能优于传统和专业的图像处理技术.

结论:

企为多重空间蛋白质组学数据预处理提供了一个简单,快速和有效的解决方案.
该工具提高了空间生物学研究的可靠性和效率.
它的直观界面有助于在现场更广泛的采用和应用.