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使用矩阵辅助激光吸附离子化质谱和新算法Quanda的治疗药物的脱氧化分析

Han Zhang¹, Yinran Xiong^2,3, Xiaonan Shi¹

¹School of Chemistry and Molecular Engineering and Research Centre of Analysis and Test, East China University of Science and Technology, Shanghai, 200237, P. R. China. wu_ting@ecust.edu.cn.

Analytical methods : advancing methods and applications

|November 19, 2024

概括

一种新的方法,QuanDA,使用质谱学量化脱化. 这种快速,具有成本效益的技术可以分析治疗药物而不需要参考标准.

科学领域:

分析化学分析化学
生物化学生物化学
药品分析药品分析

背景情况:

脱化是一种关键的翻译后修改,影响蛋白质的功能和稳定性.
精确量化自发非酶性脱化对于生物制药开发和质量控制至关重要.

研究的目的:

开发一种可靠且易于使用的方法来量化脱化.
提供一种工具来分析治疗性中的脱化,即使没有参考标准.

主要方法:

开发了QuanDA (除化量化) 方法.
使用矩阵辅助激光脱离离子化 (MALDI) 质谱法来分析同位素分布变化.
使用非负最小平方计算进行量化.

主要成果:

通过使用理论光谱,QuanDA预测模型显示出高精度 (R2 = 0.9914,RMSE = 0.03356).
该方法适用于没有对非脱化和脱化的参考标准.
仅需要的化学公式来计算同位素模式.

结论:

QuanDA 提供了一种快速,低成本和易于使用的解决方案,用于除氧化分析.
该方法有助于在治疗药物中量化除amidation,改善质量控制.
能够在各种生化和制药应用中进行准确的脱化评估.

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