人类传染病中的诊断电子显微镜 - - 方法和应用
1Centre for Biological Threats and Special Pathogens (ZBS 4), Advanced Light and Electron Microscopy, Robert Koch Institute, Berlin, Germany.
Journal of microscopy
|November 19, 2024
概括
诊断性电子显微镜 (EM) 对于在传染病中识别病原体至关重要. 本综述详细介绍了现代诊断的基本EM方法和策略,强调知识的保存.
科学领域:
- 微生物学 微生物学
- 病理学 病理学 病理学
- 显微镜的使用方法
背景情况:
- 诊断电子显微镜 (EM) 对于检测传染病中的整个病原体至关重要.
- 它的应用已经从常规转向专业诊断和病原体研究.
- 尽管病例数量正在下降,但保持对EM方法的了解至关重要.
研究的目的:
- 审查目前在传染病中诊断EM的方法和策略.
- 为了解决局限性,并在现有技术中进行改进.
- 提供示例并讨论未来的趋势,包括机器学习.
主要方法:
- 对负染色和薄切片EM技术的详细描述.
- 包括协议和最近的进步,如薄片EM用于小体积.
- 讨论样本采集,运输,保存和不活化.
主要成果:
- 已建立的EM方法 (负染色,薄切片) 仍然是核心.
- 改进和变异增强了传染病原体的诊断能力.
- 概述了样本检查和结构识别的策略.
结论:
- 诊断EM虽然在不断发展,但对于特定的传染病病例是不可或缺的.
- 保护和更新有关EM技术的知识至关重要.
- 像人工智能这样的未来进步可能会支持诊断EM应用.
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