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来自Leishmania donovani的聚胺丰富的外体驱动宿主巨细胞通过免疫代谢重编程的极化

Prince Sebastian¹, Madhulika Namdeo¹, Moodu Devender¹

¹Department of Animal Biology, School of Life Sciences, University of Hyderabad, Hyderabad 500046, India.

ACS infectious diseases

|November 19, 2024

概括

在Leishmania donovani外体中的多氨酸对于寄生虫的生存和抗药性至关重要. 这些外体细胞将巨细胞重新编程到M2状态,促进感染并创造一种抗炎环境.

科学领域:

* 寄生虫学寄生虫学
* 免疫代谢发生.
* 分子生物学 * 分子生物学

背景情况:

*Leishmania donovani (Ld) 促生菌释放含有寄生虫分子的外体,影响宿主-病原体相互作用.
* 独特的代谢成分,特别是聚胺 (PAs) 在Ld外体内在调节宿主免疫代谢中的作用尚不清楚.

研究的目的:

* 调查多胺 (PA) 在Ld外体中对寄生虫生存和健康的作用.
* 确定PAs丰富的Ld外体对巨细胞极化和功能的影响.

主要方法:

* 评估了PA抑制/减弱对L. donovani生长和健康状况的影响.
*分析了巨细胞对Ld外体的吸收.
*评估了巨细胞极化标志物 (阿基纳-1,SLC3A2) 和外体内化后的细胞内PA水平.

主要成果:

* PAs的抑制或耗尽显著损害了L. donovani的生长,特别是在耐药菌株中.
* 巨细胞有效地内化了Ld外体,增强了细胞活动和感染力.
* 内化外体诱导了M2巨细胞的两极分化,由增加的阿基因酶-1活性和SLC3A2表达体现出来,同时增加了细胞内精激素.

结论:

*多氨酸对L. donovani的生存和健康至关重要,特别是对于耐药寄生虫.
* Ld外体通过诱导M2巨细胞两极分化,促进寄生虫的生存,并为宿主的聚胺池做出贡献.
* 这些外体在巨细胞内创造了一种亲寄生,抗炎的环境,促进了感染的建立.

关键词:

抗炎药物抗炎药物抗炎药物阿尔金纳-1 的作用.外基因组是外基因组的组成部分.免疫代谢过程中的免疫代谢.巨细胞的两极分化聚氨酸是一种多氨酸.

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