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有效地准备FFPE组织样本,以保持适当的核酸质量,用于甲状腺癌的基因组分析

Yoichiro Okubo¹, Nagisa Toyama², Rika Kasajima³

¹Department of Pathology, Kanagawa Cancer Center, 2-3-2, Nakao, Asahi-Ku, Yokohama, Kanagawa, 241-8515, Japan. yoichiro0207@hotmail.com.

Endocrine pathology

|November 19, 2024

概括

这项研究提出了一种改进的方法,用于准备甲固定嵌 (FFPE) 组织样本,提高甲状腺癌基因组分析的核酸质量. 单独固定样本和淋巴结转移显示出分子分析的卓越可靠性.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
病理学病理学病理学

背景情况:

在甲状腺癌的基因组分析中,固定甲嵌入式 (FFPE) 组织至关重要,指导向治疗.
保持FFPE样本中的核酸质量对于准确的基因组分析至关重要.

研究的目的:

开发和验证一种高级技术来准备FFPE组织样本,以最大限度地提高核酸质量.
评估样品类型和制备方法对DNA和RNA完整性的影响.

主要方法:

对手术切除的甲状腺瘤,淋巴结转移和单独固定样本的分析.
优化形式素固定协议.
使用诸如DNA完整数,循环值比,RNA完整数和DV200等指标评估核酸质量.

主要成果:

与传统处理的样本相比,单独固定的瘤样本显示DNA和RNA质量明显更高.
淋巴结转移的核酸质量与甲状腺原发性瘤相当或高于甲状腺原发性瘤.
已建立的质量指标可靠地反映了核酸的保存.

结论:

准备单独固定瘤样本是保护基因组分析DNA和RNA质量的有效策略.
淋巴结转移是甲状腺癌综合基因分析的可靠来源.
开发的方法,利用活检穿孔,可以适应一般医疗实践,改善获得高质量的病理样本的机会.

关键词:

固定在形式素中的嵌入式抛.基因组分析基因组分析核酸质量的核酸质量甲状腺癌是一种甲状腺癌.

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