无形固体分散的动态相位行为通过In Situ刺激拉曼散射显微镜揭示
Teemu Tomberg1, Ilona Hämäläinen1,2, Clare J Strachan1
1Division of Pharmaceutical Chemistry and Technology, Faculty of Pharmacy, University of Helsinki, Helsinki FI-00790, Finland.
Molecular pharmaceutics
|November 19, 2024
概括
刺激拉曼散射 (SRS) 显微镜可视化在溶解过程中无形固体分散 (ASD) 中的实时化学变化. 这种技术为开发有效的ASD配方提供了关键的见解.
科学领域:
- 制药科学 制药科学
- 材料科学 材料科学 材料科学
- 化学成像技术 化学成像技术
背景情况:
- 无形固体分散 (ASD) 对于提高药物的溶解性和生物可用性至关重要.
- 了解ASD与溶解介质接触时的动态相位行为对于配方开发至关重要.
- 当前的成像技术往往缺乏必要的化学特异性和时间分辨率来捕捉这些动态过程.
研究的目的:
- 应用in situ刺激拉曼散射 (SRS) 显微镜来实时,化学特定的 ASD 动态相现象的成像.
- 量化分析ASDs的行为,包括药物,聚合物和水分发,在溶解过程中.
- 提供关于ASD中水诱导的阶段过渡和药物释放机制的见解.
主要方法:
- 使用SRS显微镜,用于动态成像的快速光谱聚焦.
- 采用SRS光谱的多变量分离,用于对组件分布的半定量分析.
- 集成的关联和频率生成和共聚焦反射显微镜用于增强相位和晶度灵敏度.
主要成果:
- 实时成像揭示了不同药物负载 (20-60% w/w) 的ASD的水透,面向和散装相分离.
- 药物加载和相位依赖的药物和聚合物释放行为被定量可视化.
- 在20%药物负载的自闭症患者中观察到液-液相分离.
结论:
- 具有快速光谱聚焦的SRS显微镜可以提供高分辨率的定量洞察力,了解ASD中水诱导的相位现象.
- 该技术能够同时实现动态过程的化学,固态,时间和空间分辨率.
- 这些发现对于优化ASD配方设计和预测药物释放性能至关重要.
更多相关视频
06:54Characterization of Nanocrystal Size Distribution using Raman Spectroscopy with a Multi-particle Phonon Confinement Model
Published on: August 22, 2015
13.5K
06:26Orientational Transition in a Liquid Crystal Triggered by the Thermodynamic Growth of Interfacial Wetting Sheets
Published on: May 15, 2017
7.1K
相关概念视频
01:20Raman Spectroscopy: Overview
311
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
311
01:10X-ray Diffraction of Biological Samples
3.8K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are scattered by the electron clouds around the sample atoms. The X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
3.8K