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使用双触发MOF-Fe (DTNB) 进行强大而敏感的色度检测

Kaining Zhang¹, Hao Sun², Long Wei²

¹Shandong Provincial Hospital Affiliated to Shandong First Medical University, Ji'nan, Shandong 250021, PR China.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy

|November 19, 2024

概括

使用MOF-Fe (DTNB) 的新双触发色度法提供了精确而敏感的谷氨 (GSH) 检测. 这种简化方法增强了对各种健康状况的生物化学分析和临床检测.

科学领域:

生物化学分析生物化学分析
材料科学是一种材料科学.
分析化学是一种分析化学.

背景情况:

谷氨 (GSH) 水平是包括癌症,心脏病和衰老在内的生理和病理状态的关键生物标志物.
准确而敏感的GSH检测方法对于临床诊断和研究来说是非常理想的.
现有的GSH检测方法可能受到敏感性,选择性或复杂性的限制.

研究的目的:

开发一种新的,强大的,敏感的色度测量方法来检测谷.
使用基于金属有机框架 (MOF-Fe(DTNB)) 的双触发机制.
为了验证该方法在生物血样本中的性能.

主要方法:

合成了一种具有过氧化酶类活性的新型金属有机框架 (MOF-Fe(DTNB)).
该方法采用双触发机制,涉及通过GSH的降解作用和醇交换抑制TMB氧化.
氧化TMB (ox-TMB) 的色度检测使用光谱测量进行.

主要成果:

基于MOF-Fe (DTNB) 的方法表明高过氧化酶活性,催化TMB氧化.
双触发机制允许高精度 (RSD <6%) 和灵敏度 (LOD = 0.28μM) 的稳健的GSH度测量.
该方法在生物血样本中显示出令人满意的恢复率 (96.7-103.3%),干扰最小.

结论:

开发的双触发MOF-Fe (DTNB) 色度策略为GSH检测提供了简化和准确的方法.
该方法具有出色的灵敏度,选择性和稳定性,适合生物化学分析.
由于该技术的简单性和较少的仪器仪表要求,该技术在护理点测试应用中具有重大潜力.

关键词:

颜色测量试验的测定方法双触发式触发方式葡萄糖氨酸 (Glutathione) 是一种金属有机框架的框架.过氧化酶的使用

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