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Updated: Jun 7, 2025

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells
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Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Published on: July 30, 2020

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使用基于mTurquoise2的平台开发ATP,cAMP,酸盐和葡萄糖的光寿命生物传感器

Chongxia Zhong1, Satoshi Arai2, Yasushi Okada3

  • 1WPI Nano Life Science Institute, Kanazawa University, Kakuma-machi, Kanazawa 920-1192, Japan; Laboratory for Cell Polarity Regulation, RIKEN Center for Biosystems Dynamics Research (BDR), Suita, Osaka 565-0874, Japan.

Cell reports methods
|November 19, 2024
PubMed
概括

研究人员开发了新的基于单个光蛋白 (FP) 的FLIM生物传感器,用于可视化细胞过程. 这种多功能的mTurquoise2 (mTQ2) 平台简化了查,并使双重成像能够改善定量细胞内分析.

关键词:
这就是ATPATPATP ATP.CP: 图像处理 图像处理飞行员飞行员的飞行员生物传感器生物传感器在CAMP中,我们可以使用cAMP.酸是一种酸.光终身成像显微镜显微镜光蛋白是一种光蛋白质.葡萄糖 葡萄糖 葡萄糖 是 一种定量成像技术 定量成像技术

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Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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