Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

8.7K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
8.7K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

7.2K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
7.2K
Non-LTR Retrotransposons03:18

Non-LTR Retrotransposons

11.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
11.4K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

The ITCC-P4 PDX Platform Enables Preclinical Testing of Pediatric Cancers.

Cancer research·2026
Same author

Statescope: an integrative deconvolution framework for discovering cell states in tumors.

Nature communications·2026
Same author

Live Attenuated Influenza Virus as a Vector for Multivalent T-Cell Vaccines: Targeting RSV, hMPV, and PIV3.

Vaccines·2026
Same author

Surgical Removal of Residual Tumor Masses in Patients Undergoing Targeted Therapy for EGFR-Mutated Locally Advanced or Metastatic Lung Cancer.

International journal of cancer·2026
Same author

Network methods for diagonal integration of unpaired single-cell multiomics data: a review.

Bioinformatics (Oxford, England)·2026
Same author

Identifying the Aggression Impulsive/Reactive (AIR) Profile in Youth With Behavioral Challenges.

JAACAP open·2026

相关实验视频

Updated: Jun 7, 2025

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

11.1K

确定Wnt驱动的长非编码转录组中的结直肠癌特异性漏洞.

Laura J Schwarzmueller1,2, Ronja S Adam1,2, Leandro F Moreno1,2

  • 1Laboratory for Experimental Oncology and Radiobiology, Center for Experimental and Molecular Medicine, Cancer Center Amsterdam, Amsterdam UMC, Amsterdam, The Netherlands.

Gut
|November 19, 2024
PubMed
概括

异常的Wnt通路激活驱动着结直肠癌 (CRC). 研究人员确定了一种新的长非编码RNA,LINC02418,对CRC干细胞功能和瘤生长至关重要,提供了新的治疗点.

关键词:
结肠直肠癌是什么意思

更多相关视频

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
10:33

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

11.0K
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
06:52

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

6.5K

相关实验视频

Last Updated: Jun 7, 2025

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells
10:13

A Multiplexed Luciferase-based Screening Platform for Interrogating Cancer-associated Signal Transduction in Cultured Cells

Published on: July 3, 2013

11.1K
Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts
10:33

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

11.0K
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres
06:52

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

6.5K

科学领域:

  • 在瘤学瘤学.
  • 分子生物学分子生物学
  • 遗传学 是一个遗传学.

背景情况:

  • 异常的Wnt通路信号传递是结直肠癌 (CRC) 发病的一个关键因素,推动了瘤的进展.
  • 虽然Wnt级联的蛋白质编码标已知,但长非编码RNAs (lncRNAs) 在CRC中的作用仍然不完全理解.

研究的目的:

  • 在结直肠癌中全面映射Wnt调节的lncRNA转录组.
  • 识别和验证必要的lncRNAs作为CRC的潜在治疗点.

主要方法:

  • 利用全球运行测序来识别长非编码RNAs (lncRNAs),这些RNAs由CRC中的β-catenin调节.
  • 采用CRISPR干扰 (CRISPRi) 脱落屏幕来评估特定lncRNAs在CRC细胞扩张中的功能重要性.

主要成果:

  • 发现LINC02418对于结直肠癌细胞的克隆生长至关重要.
  • 阐明了LINC02418通过调节MYC表达和抑制终端分化来促进CRC干细胞特征.
  • 开发了有效的小干扰RNA (siRNA) 疗法,针对LINC02418进行体内应用.

结论:

  • 针对癌症的Wnt调节的IncRNAs为向Wnt通路提供了有前途的治疗途径.
  • LINC02418代表了结直肠癌的潜在新型治疗标,解决了当前Wnt通路抑制策略的局限性.