通过酶提取的乌尔万通过STING信号传递和I型干扰素限制病毒,在细胞进入后
Meifang Zhang1, Wenwei Han2, Leke Qiao3
1Key Laboratory of Marine Drugs of Ministry of Education, School of Medicine and Pharmacy, Ocean University of China, Qingdao 266003, China; Key Laboratory of Tropical Biological Resources of Ministry of Education and One Health Institute, School of Pharmaceutical Sciences, Hainan University, Haikou 570228, China.
Carbohydrate polymers
|November 19, 2024
概括
来自Ulva prolifera的Ulvan多糖体通过激活细胞免疫路径,表现出强大的抗病毒活性. 一种特定的乌尔万PR1抑制了囊泡性口炎病毒,并通过一种依赖能量的机制进入细胞,这表明一种新的治疗方法.
科学领域:
- 海洋生物技术 海洋生物技术
- 免疫学 免疫学 免疫学
- 病毒学 病毒学
背景情况:
- 来自Ulvales藻类的Ulvan多糖类具有已知的抗病毒特性.
- 乌尔旺抗病毒活性背后的精确机制在很大程度上仍未被探索.
- 了解Ulvan与宿主细胞的相互作用对于治疗开发至关重要.
研究的目的:
- 为了研究来自Ulva prolifera的Ulvan部分的抗病毒机制.
- 描述特定的乌尔万多糖和寡糖的抗病毒活性和细胞吸收.
- 阐明细胞内信号通路在介导免疫反应中的作用.
主要方法:
- 从Ulva prolifera中酶提取和净化乌尔瓦分数 (PR1,PR2,PR3).
- 乌尔旺分数的结构和单糖化合物组成分析.
- 在巨细胞和细胞系中使用囊泡性口腔炎病毒 (VSV) 的抗病毒测定 (A549,Vero).
- 对细胞吸收机制和细胞内信号通路的研究 (STING,p65,IRF3).
主要成果:
- PR1 (13.5 kDa) 在巨细胞中显著抑制了VSV (IC50 = 179.1 ng/mL) 和在A549细胞中表现出中度活性.
- PR1的抗病毒作用依赖于I型干扰素,因为它在缺乏IFN的Vero细胞中无效.
- PR1表现出对能量依赖的细胞进入,这对于乌尔瓦人来说是一个新发现.
- 乌尔万分数激活了细胞内STING信号通路,导致p65和IRF3酸化和核转移.
结论:
- 乌尔万多糖,特别是PR1,具有通过I型干扰素介导的显著抗病毒特性.
- PR1的依赖能源的细胞吸收代表了乌尔万的新发现特征.
- 突发性激活STING通路提供了对其免疫调节功能的新见解.
- 这些发现突出了乌尔万作为抗病毒药物的潜力,并强调了细胞内机制在它们的作用中的重要性.
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