工程学Saccharomyces boulardii用于l-ergothioneine的益生菌补充剂
Chaoqun Tang1,2, Lu Zhang1, Junyi Wang1
1State Key Laboratory of Cellular Stress Biology, School of Life Sciences, Faculty of Medicine and Life Sciences, Xiamen University, Fujian, China.
Biotechnology journal
|November 19, 2024
概括
研究人员设计了Saccharomyces boulardii,一种益生菌酵母,以产生抗氧化剂l-ergothioneine (EGT). 这种基因修饰增强了酵母的功效.
科学领域:
- 微生物学 微生物学
- 基因工程是一种基因工程.
- 生物技术是生物技术.
背景情况:
- 萨卡罗米塞斯 (Saccharomyces boulardii) 是一种益生菌酵母,已知对肠道健康和胃肠道疾病有好处.
- 它的抗微生物和免疫调节特性使其成为益生菌的关键研究对象.
- 提高其抗氧化能力可以扩大其治疗应用.
研究的目的:
- 通过使l-ergothioneine (EGT) 的产生能够改善Saccharomyces boulardii的抗氧化特性.
- 建立一个有效的基因组编辑系统S. boulardii使用PiggyBac转位子系统.
- 开发新的EGT产生益生菌菌株,用于潜在的治疗用途.
主要方法:
- 在S. boulardii中构建了一个双重淘汰基因 (ura3和trp1基因),用于基于等离子体的表达.
- 实施了PiggyBac转位子系统,以稳定地将异质基因表达盒集成到S. boulardii基因组中.
- 利用增强的绿色光蛋白 (EGFP) 作为记者基因来确认染色体的整合.
主要成果:
- 使用PiggyBac系统成功实现了EGFP表达盒的随机染色体集成.
- 产生了多样化的EGT产生的S. boulardii菌株,超越了传统的基因组编辑限制.
- 鉴定出一种产量最高的菌株,在120小时的栽培后,其产量为17.50毫克/升EGT.
结论:
- 在S. boulardii中首次成功应用了PiggyBac转位子系统,使基因工程成为可能.
- 工程S. boulardii现在通过EGT生产具有增强的抗氧化特性.
- 这些工程益生菌具有针对胃肠道疾病的新疗法和食补充剂的潜力.
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