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相关概念视频

CRISPR01:59

CRISPR

49.5K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
49.5K
CRISPR and crRNAs02:53

CRISPR and crRNAs

16.7K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
16.7K
Homologous Recombination02:31

Homologous Recombination

50.2K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.2K
Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
5.9K

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相关实验视频

Updated: Jun 7, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
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Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

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通过基础模型发现具有自我处理crRNA前能力的CRISPR-Cas系统.

Wenhui Li1,2,3, Xianyue Jiang3, Wuke Wang3

  • 1State Key Laboratory of Cardiology and Medical Innovation Center, Shanghai East Hospital, Frontier Science Center for Stem Cell Research, Bioinformatics Department, School of Life Sciences and Technology, Tongji University, Shanghai, China.

Nature communications
|November 20, 2024
PubMed
概括

本研究介绍了CHOOSER,这是一种用于发现CRISPR-Cas系统的AI框架. 它确定了11种新的Casλ同类物种,包括一种具有自我处理能力的潜在病原体检测物种.

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Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
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相关实验视频

Last Updated: Jun 7, 2025

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科学领域:

  • 遗传学和基因组学 遗传学和基因组学
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 克里斯普尔-卡斯系统是强大的基因编辑工具.
  • 传统的发现方法错过了遥远的同类,缺乏功能识别.
  • 蛋白质大语言模型 (LLM) 为Cas系统建模提供了新的可能性.

研究的目的:

  • 开发一个人工智能框架,用于无对齐地发现CRISPR-Cas系统.
  • 识别具有自我处理crRNA前能力的新型CRISPR-Cas同类.
  • 探索这些系统在基因编辑和诊断方面的潜力.

主要方法:

  • 使用了CHOOSER (Cas HOmlog观察和自我处理扫描),这是一个使用蛋白质基础模型的人工智能框架.
  • 对CRISPR-Cas系统进行了无对齐选.
  • 用于对已识别的同类进行实验验证.

主要成果:

  • 确定了11个Casλ同类,几乎是已知的目录的两倍.
  • 发现并经过实验验证的EphcCasλ用于自处理crRNA前,DNA裂变和跨裂变.
  • 证明了CHOOSER在发现功能CRISPR-Cas系统中的有效性.

结论:

  • CHOOSER提供了一种创新的方法,用于发现具有特定功能的CRISPR-Cas系统.
  • 已识别的EphcCasλ同源对基于CRISPR的病原体检测有显著的前景.
  • 这项工作扩展了CRISPR-Cas系统的目录,并突出了它们在先进生物技术应用中的潜力.