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多菌株益生菌的使用降低了死角性肠球炎的严重程度,并改变了小鼠的表观遗传特征

Daphne H Klerk^1,2, Hannah Moore¹, Daniel J Scheese¹

¹Division of Pediatric Surgery, Department of Surgery, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Pediatric research

|November 20, 2024

概括

多菌株益生菌显著改变了肠道表观遗传学,降低了死性肠球炎 (NEC) 的严重程度,并在小鼠模型中改善了肠道运动性. 这些发现揭示了新型表观遗传机制,即NEC中益生菌的益处.

科学领域:

微生物学和免疫学
胃肠病学胃肠病学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

结核性肠球炎 (NEC) 是新生儿的一种严重的肠道疾病,其益生菌预防机制尚不清楚.
研究益生菌对肠道表观遗传学,炎症和运动性的影响,对于理解NEC病原和治疗至关重要.

研究的目的:

为了研究多菌株益生菌对肠道表观遗传学在NEC的小鼠模型的影响.
评估益生菌对肠道炎症和动力的影响,以大肠主导和大肠结肠炎NEC模型.

主要方法:

在C57BL/6J小鼠中,从出生后3-11天开始,给予多菌株益生菌 (B.infantis,B.lactis,S.thermophilus).
从产后第8天开始使用配方,NEC细菌和0.5%的DSS诱导NEC.
量化了DNA甲基化,胃肠道过渡时间和益生菌殖民.

主要成果:

益生菌的使用引起了小肠表观遗传特征的显著变化.
益生菌降低了NEC的严重程度,改善了肠道运动性,在胆结肠炎模型中效果更为明显.
在接受治疗的小鼠中确认了益生菌殖民.

结论:

多菌株益生菌在肠道粘膜中施加表观遗传修饰.
益生菌可以减轻NEC诱导的表观遗传变化,并减少疾病的严重程度.
这些发现为临床相关的NEC模型中的益生菌作用机制提供了洞察力.

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