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Updated: Jun 7, 2025

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
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Published on: February 7, 2019

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一个全性转录因子的高度多重设计,以感知新的配体

Kyle K Nishikawa1, Jackie Chen1, Justin F Acheson1

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI, USA.

Nature communications
|November 20, 2024
PubMed
概括

研究人员开发了Sensor-seq,这是一个高通量平台,用于为新型分子设计全转录因子 (aTF) 生物传感器. 这种方法成功地为各种非原生配体创建了生物传感器,使分子检测中的新应用成为可能.

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