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CmPYL7通过与东方西瓜中的CmPP2C24-like相互作用来积极调节寒冷耐受性

Wei Liu¹, Yun Jiang², Yanling Lv¹

¹Vegetable Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, Liaoning, PR China.

Physiologia plantarum

|November 20, 2024

概括

CmPYL7通过与CmPP2C24相似的相互作用来积极调节东方瓜的寒冷耐受性,增强抗氧化活性和ABA信号传递. 沉默CmPYL7降低了寒冷耐受性,而过度表达增加了它.

科学领域:

植物生物学植物生物学
分子生物学分子生物学
压力生理学压力生理学

背景情况:

酸 (ABA) 受体 (PYR/PYL/RCARs,PYLs) 在ABA信号传递中至关重要.
之前发现,CmPYL7在东方瓜 (Cucumis melo) 中被冷应激调高.

研究的目的:

调查CmPYL7在东方瓜的寒冷耐受性中的作用.
阐明CmPYL7介导的冷反应的分子机制.

主要方法:

在各种压力条件下的基因表达分析 (寒冷,ABA,fluridone).
基因沉默 (使用TRV) 和过度表达在东方西瓜和Arabidopsis.
测量生理参数:抗氧化酶活性 (SOD,CAT,APX),H2O2含量,电解质泄漏,MDA,プロ林和可溶糖含量.
蛋白质与蛋白质相互作用测试 (体内和体外).

主要成果:

CmPYL7表达是由寒冷,ABA和在寒冷压力下的ABA抑制诱导的.
沉默CmPYL7降低了寒冷耐受性,而过度表达增强了它,影响了抗氧化酶活动和保护性化合物积累.
CmPYL7与类似于CmPP2C24的寒冷耐受性的负调节剂相互作用.
沉默CmPP2C24-like增强的耐寒性和抗氧化能力.

结论:

CmPYL7在ABA信号通路中发挥积极作用,以增强耐寒性.
CmPYL7与CmPP2C24-like相互作用,这表明冷应激反应中的调节机制.

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