过高homocysteinemia对卵泡发育和卵子质量的影响
Lu Wang1, Jinmei Gao1, Jie Ma1
1Ningxia Medical University, General Hospital of Ningxia Medical University, Ningxia, China.
iScience
|November 20, 2024
概括
高水平的homocysteine (HHcy),即使没有PCOS,通过降低卵细胞质量和卵巢功能,也会损害女性生育能力. 这通过线粒体功能障碍和 zona pellucida 异常影响生殖寿命.
科学领域:
- 生殖生物学 生殖生物学
- 内分泌学 在内分泌学.
- 线粒体医学是指线粒体医学.
背景情况:
- 多囊性卵巢综合征 (PCOS) 与卵泡液中同类氨酸 (Hcy) 的升高有关,这会对卵细胞和胚胎质量产生负面影响.
- 过高homocysteinemia (HHcy) 对没有PCOS的个体生殖功能的特定影响尚不清楚.
研究的目的:
- 在小鼠模型中研究超同胞蛋白血症 (HHcy) 对女性生殖功能和卵细胞质量的影响.
- 为了确定HHcy是否影响生育能力,卵巢发育,卵细胞成熟和早期胚胎发育,独立于PCOS.
主要方法:
- 使用了一种超同胞蛋白血症 (HHcy) 的小鼠模型.
- 评估生育率,卵巢组织学 (动卵泡) 和卵细胞成熟.
- 对卵细胞进行蛋白质组分析,以确定分子变化.
- 使用传输电子显微镜进行卵细胞的超结构检查.
- 进行体外受精 (IVF) 来评估胚胎早期发育.
主要成果:
- HHcy小鼠的生育能力降低,卵巢动卵泡增加,卵细胞成熟率降低.
- 蛋白质组分析揭示了线粒体功能障碍和HHcy小鼠卵细胞中 zona pellucida蛋白 (ZP1,ZP2,ZP3) 的表达减少.
- 电子显微镜显示HHcy卵细胞中异常的 zona pellucida 形成和微.
- 试管受精导致HHcy小鼠的2细胞胚胎形成率降低.
结论:
- 超同胞蛋白血症 (HHcy) 对女性生殖寿命产生负面影响.
- HHcy会影响卵泡发育和卵细胞质量,导致生育能力下降.
- 线粒体功能障碍和光区异常是HHcy损害生殖功能的关键机制.
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