Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

[Quality evaluation of Asparagi Radix based on saponin fingerprinting and content determination].

Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica·2026
Same author

Predicting Daily Cardiovascular Emergencies Using Weather and Air Quality Data: A 23-Year Machine-Learning Analysis in Taiwan.

GeoHealth·2026
Same author

Topical Ascorbic Acid for Corneal Endothelial Cell Density Enhancement, Protection, and Recovery in Fuchs Dystrophy After Complicated Cataract Surgery.

Eye & contact lens·2026
Same author

Transcriptomic analysis suggests ferroptosis as a potential mechanism of fipronil-induced cytotoxicity in human glioblastoma cells.

Toxicology in vitro : an international journal published in association with BIBRA·2026
Same author

Hydrophilicity-Enhanced NH<sub>2</sub>-MIL-88B(Fe) Integrated Photocatalytic Membrane Reactor for Simultaneous Rejection and Degradation of Low-Density Polyethylene in Water Matrices.

ACS applied materials & interfaces·2026
Same author

Liuwei Dihuang pills ameliorate renal injury in experimental type 2 diabetes mellitus rat by regulating host-gut microbiota interaction.

Frontiers in pharmacology·2026

相关实验视频

Updated: Jun 7, 2025

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

60.3K

免疫细胞循环-ID - - 一种基于高精度免疫光的方法,用于细胞循环识别.

Yu-Lin Chen1, Yu-Chia Chen1,2, Aussie Suzuki1,2,3

  • 1McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin-Madison, Madison, Wisconsin, 53705, USA.

Journal of cell science
|November 20, 2024
PubMed
概括
此摘要是机器生成的。

一种新的免疫光方法ImmunoCellCycle-ID,精确地识别单细胞水平的细胞周期阶段. 这种用户友好的技术提供了详细的细胞循环分析作为流细胞计的替代方案.

关键词:
细胞循环的细胞周期图像成像是一种成像.基于免疫光的细胞周期识别.显微镜的使用方法

更多相关视频

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.2K
Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
09:57

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

12.9K

相关实验视频

Last Updated: Jun 7, 2025

Analysis of Cell Cycle Position in Mammalian Cells
12:19

Analysis of Cell Cycle Position in Mammalian Cells

Published on: January 21, 2012

60.3K
Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes
09:39

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

15.2K
Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software
09:57

Workflow for High-content, Individual Cell Quantification of Fluorescent Markers from Universal Microscope Data, Supported by Open Source Software

Published on: December 16, 2014

12.9K

科学领域:

  • 细胞生物学 细胞生物学
  • 分子生物学分子生物学

背景情况:

  • 细胞周期包括G1,S,G2和M阶段,对于细胞增殖和分化至关重要.
  • 调节细胞循环的蛋白质对于癌症的预防和进展至关重要.
  • 像流细胞计这样的传统方法需要大量的细胞数量来进行准确的细胞周期分析.

研究的目的:

  • 开发一种用户友好的单细胞分辨率方法,用于精确识别细胞周期阶段.
  • 为详细的细胞循环分析提供流细胞计的替代或补充方法.

主要方法:

  • 开发一种以免疫光为基础的检测方法,命名为ImmunoCellCycle-ID.
  • 使用光显微镜精确识别G1,S,G2和M阶段,包括子阶段.

主要成果:

  • 免疫细胞周期-ID提供单细胞分辨率用于细胞周期阶段识别.
  • 该方法准确地区分G1,早期/中期S,晚期S,早期/中期G2,晚期G2和M期子阶段.
  • 在各种细胞系中实现了高精度的细胞周期识别.

结论:

  • 免疫细胞循环-ID 是一个有价值的工具,用于单细胞水平的详细细胞循环分析.
  • 这种方法可以补充或取代传统的流细胞计,用于剖析细胞周期子阶段.
  • 该技术增强了对不同细胞类型细胞循环调节的研究.