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Rous Sarcoma Virus (RSV) and Cancer01:03

Rous Sarcoma Virus (RSV) and Cancer

Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...

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cds46,一种高度可变的鱼水病毒基因.

Laetitia Montacq1, Doriana Flores2, Hélène Giummarra2

  • 1IHAP, Université de Toulouse, INRAE, ENVT, 31300 Toulouse, France.

The Journal of general virology
|November 20, 2024
PubMed
概括

水腫病毒 (CEV) 的基因多樣性仍然不清楚. 确定了一个新的基因组区域,cds46,提供了对CEV演变的见解,以及跟踪不同病毒菌株的潜力.

关键词:
这就是Poxviridae.鱼类鱼是什么样的鱼类流行病学流行病学睡眠疾病是一种睡眠疾病.

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科学领域:

  • 病毒学 病毒学
  • 水生动物卫生 水生动物卫生
  • 基因组学就是基因组学.

背景情况:

  • 虫水腫病毒病 (CEVD) 在魚和魚群體中造成重大經濟損失.
  • 鱼水病毒 (CEV) 的遗传多样性和进化,一个Poxviridae家族的成员,是不太了解.
  • 之前的研究根据4a基因片段确定了两个基因组 (gI和gII).

研究的目的:

  • 为了更好地了解其遗传多样性和进化,研究CEV中的新型基因组区域.
  • 为了探索新发现的开放阅读框架 (ORF) 的潜在功能和起源,cds46.
  • 评估这个新的基因组区域对于识别和跟踪CEV单元型的有用性.

主要方法:

  • 对最近的CEV样本进行分析,以确定可变的基因组区域.
  • 生物信息分析,包括使用AlphaFold进行ORF预测和蛋白质结构预测.
  • 进行比较基因组学,分析不同CEV基因组和地理来源的cds46位点的变异.

主要成果:

  • 一个新的基因组区域,编码cds46ORF,被确定具有显著的变异 (替代,indels,重组).
  • 阿尔法折叠分析表明cds46编码的蛋白质可能作为细胞内核酶起作用,为其起源提供线索.
  • 在基因组I (gI) 和一些基因组II (gII) 单元型中缺少cds46ORF;gI单元型具有独特的121 bp插入.
  • 在cds46位点内观察到两个不同的等位基组,通过替代物进行区分.
  • 在来自法国,日本和以色列的鱼群中检测到混合CEV单元型.
  • 一个2023年的样本中含有CEV,其gII-like4a等位基因曾在2015年在法国被发现,这表明持续传播.

结论:

  • cds46位点代表了CEV中的新型和可变的基因组区域,对于理解其演变至关重要.
  • cds46的潜在内核酶功能为研究CEV生物学提供了新的途径.
  • 在全球范围内,cds46位点作为一种有价值的遗传标记,用于区分和跟踪CEV单元型.