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使用异热模型和线性方程,对菌毒素结合剂与脱氧尼瓦伦醇和烟素B₁的评估

Luara Medianeira de Lima Schlösser¹, Dison Stracke Pfingsten Franco², Janine Alves Sarturi¹

¹Laboratory of Mycotoxicological Analyses, Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.

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概括

两个真菌毒素结合剂被评估了它们吸附脱氧尼瓦伦醇 (DON) 和烟素B1 (FB1) 的能力. 吸附异热模型提供了可靠的数据,BBAc显示了比SepHt更高的DON吸附率.

科学领域:

动物科学动物科学
食品科学食品科学食品科学
环境科学环境科学

背景情况:

像脱氧尼瓦伦醇 (DON) 和烟素B1 (FB1) 这样的真菌毒素对动物健康和食品安全构成重大风险.
有效的真菌毒素结合剂对于减轻动物料和食品中的这些风险至关重要.

研究的目的:

评估两种真菌毒素结合剂BBAc和SepHt对DON和FB1的*体外*吸附特性.
在不同的pH条件下比较BBAc (本托尼特,β-葡萄糖,活性炭) 和SepHt (热处理的 sepiolite) 的疗效.
为了确定最合适的吸附异热模型来预测粘合剂性能.

主要方法:

两种真菌毒素结合剂BBAc和SepHt在0.5%度下对DON和FB1 (0.5-10毫克L-1) 进行了测试.
在pH 3和pH 6的溶液中*in vitro*评估吸附性.
应用了Brunaeur-Emmett-Teller,Dubinin-Radushkevich,Freundlich和Langmuir等热模型,并通过LC-MS/MS分析了数据.

主要成果:

富蒙尼辛B1吸附率在两个pH值下BBAc和SepHt之间没有显著差异.
在1毫克L-1.1度下,BBAc表现出明显高于SepHt的脱氧尼瓦伦醇吸附率,比SepHt更高.
弗洛伊德利希模型最好地描述了BBAc的DON吸附和BBAc和FB1的吸附,在pH3时对两个结合剂都是FB1吸附. 兰穆尔模型最适合在pH 6.0下对DON的SepHt和FB1的两个结合剂.

结论:

吸附异热模型,特别是弗洛恩德利希和兰格穆尔,为评估菌毒素结合剂提供比线性回归方程更可靠和更有信息的数据.
结合剂的选择和特定的真菌毒素可以影响基于pH的最佳吸附模型和性能.
在测试条件下,与SepHt相比,BBAc显示了增强脱氧尼瓦伦醇结合的潜力.

关键词:

脱氧尼瓦伦 (deoxynivalenol) 是一种除氧化.在HPLC-MS/MS中使用.用于料添加剂的料添加剂富莫尼辛 B1 的使用.在体外 (in vitro) 的情况下.

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