在牛奶产品样本中用液-液微提取程序染色学测定牛氧三环素
Ilona Kiszkiel-Taudul1, Martyna Skorupska1
1Chemical Department, University of Bialystok, Bialystok, Poland.
概括
这项研究介绍了一种小型化液体液体微提取 (LLME) 方法,使用butanol选择性地分离乳制品中的氧基环素 (OTC) 抗生素残留物. 开发的技术可以在监管层面准确地进行OTC检测.
科学领域:
- 分析化学 分析化学
- 食品安全 食品安全
- 环境科学 环境科学
背景情况:
- 微型化的分析程序减少了有机溶剂的使用.
- 氧基环素 (OTC) 是一种经常在乳制品中发现的抗生素.
- 准确确定OTC对于食品安全和监管合规性至关重要.
研究的目的:
- 开发和优化液体-液体微提取 (LLME) 方法,用于选择性地隔离OTC.
- 为了在样本脱蛋白化后为LLME建立最佳条件.
- 通过HPLC-UV和LC-MS/MS验证乳制品中OTC测定方法.
主要方法:
- 液体-液体微提取 (LLME) 使用醇作为提取剂.
- 在提取之前样品的脱蛋白化.
- 使用高性能液体染色学与紫外线检测 (HPLC-UV) 和液体染色学-双重质谱法 (LC-MS/MS) 分析提取物.
主要成果:
- 通过使用700μL的butanol,确定了最佳的LLME条件.
- 分析物的恢复率很高,从97.6%到98.9%不等.
- 达到适用于在最大残留量限制 (MRL) 水平下OTC检测的检测极限,LC-MS/MS提供更高的灵敏度 (2.75μg kg-1).
结论:
- 开发的LLME方法对从乳制品基质中选择性OTC隔离有效.
- 该方法表现出良好的精度 (1.7%-8.6%) 和高回收率,适合常规分析.
- 经过验证的方法允许可靠地检测和量化牛奶和小奶酪中的OTC残留物.
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