蛋白酸酶1催化子单元玛是导致肉类轻度和红色的基因
Jiahong Sun1, Xinting Yang1, Guiping Zhao1
1State Key Laboratory of Animal Biotech Breeding, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Key Laboratory of Animal (Poultry) Genetics Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Beijing, China.
PLoS genetics
|November 20, 2024
概括
蛋白酸酶1催化子单元 (PPP1CC) 被确定为影响肉色的关键基因. 它的抑制增加了肉的轻度,并改变了肌肉纤维类型,为改善肉质提供了新的途径.
科学领域:
- 动物遗传学和动物基因组学
- 肉类科学和肌肉生物学
背景情况:
- 肉质量对消费者至关重要,颜色 (轻度,红色,黄色) 是主要指标.
- 识别影响肉色的遗传因素对于通过育种和技术提高肉质量至关重要.
研究的目的:
- 通过全基因组关联研究 (GWAS) 来识别与肉轻度和红色相关的基因.
- 阐明已识别的基因在调节肉色和肌肉特征方面的功能作用.
主要方法:
- 全基因组关联研究 (GWAS) 和基于基因的关联分析对230只快速生长的白色羽毛进行.
- 门德尔随机化,可诱导的骨肌特异性淘汰 (PPP1CC-SSKO) 鼠标生成,转录组和代谢组分析.
- 等离子体记者测试以验证SNP调节功能和品种之间的比较分析.
主要成果:
- 蛋白酸酶1催化子单元 (PPP1CC) 被确定为肉色的致病基因,与轻度和红色有显著的关联.
- 在小鼠中抑制PPPP1CC导致肉的轻度增加,肌球蛋白减少,并从慢肌纤维转变为快肌纤维.
- PPP1CC中的特定SNP (rs315520807) 调节基因转录,其频率在品种之间有所不同,与肉色差异相关.
结论:
- 证实PPP1CC是控制肉颜色的关键基因.
- 该研究确定了一种新的基因 (PPP1CC) 和一种调控变异 (rs315520807) 用于肉色,为肉类改进技术提供了有价值的目标.
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